CEACAM3-Specific Polyclonal antibody

CEACAM3-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19496-1-AP

Synonyms

CEACAM3, W264, CGM1, CD66D, Carcinoembryonic antigen-related cell adhesion molecule 3

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Validation Data Gallery

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  IHC staining of human colon cancer using 19496-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19496-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 19496-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 19496-1-AP (CEACAM3-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

19496-1-AP targets CEACAM3-Specific in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: carcinoembryonic antigen-related cell adhesion molecule 3
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: NM_001815
• Gene Symbol: CEACAM3
• Gene ID (NCBI): 1084
• RRID: AB_2878582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEACAM3, also named as CD66D and CGM1, belongs to the immunoglobulin superfamily and CEA family. CEACAM3 is the major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species. CEACAM3 plays an important role in the clearance of pathogens by the innate immune system. It is responsible for RAC1 stimulation in the course of pathogen phagocytosis. The antibody is specific to isoform1 of CEACAM3.

Publications

Species	Application	Title
mouse	IHC	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
human	IHC,IF	Cell Biosci; Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.; Authors - Jiaqi Jin