Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 13280-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13280-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 13280-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 13280-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of Neuro-2a using 13280-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CEND1 antibody (13280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CEND1 antibody (13280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, SH-SY5Y cells, human brain tissue, rat brain tissue
Positive IHC detected in
mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Neuro-2a cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cell cycle exit and neuronal differentiation 1 (CEND1) also known as BM88, is a neuronal protein associated in vivo with terminal neuron-generating divisions, marking the exit of proliferative cells from the cell cycle (PMID: 17971443). CEND1 expression is critical for the NEUROG2-driven reprogramming of astrocytes, suggesting the existence of a reciprocal feedback loop leading to neurogenesis (PMID: 26321141). CEND1 is an integral membrane protein composed of two 23-kDa polypeptide chains linked together by disulfide bridges.
The Protective Effects and Mechanisms of Essential oil from Chimonanthus nitens Oliv. Leaves in Allergic Rhinitis Based on the NF-κB and T-bet/GATA-3 signaling pathways
Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 13280-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13280-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13280-1-AP (CEND1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 13280-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 13280-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13280-1-AP (CEND1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of Neuro-2a using 13280-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CEND1 antibody (13280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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