Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | rat small intestine tissue, human lung cancer tissue, mouse liver tissue, mouse kidney tissue, human pancreas cancer tissue, mouse small intestine tissue, rat skin tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28568-1-AP targets CENPF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29665 Product name: Recombinant human CENPF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2915-3114 aa of NM_016343 Sequence: SVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ Predict reactive species |
| Full Name | centromere protein F, 350/400ka (mitosin) |
| Calculated Molecular Weight | 368 kDa |
| Observed Molecular Weight | 350 kDa |
| GenBank Accession Number | NM_016343 |
| Gene Symbol | CENPF |
| Gene ID (NCBI) | 1063 |
| RRID | AB_2918176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49454 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CENPF antibody 28568-1-AP | Download protocol |
| IHC protocol for CENPF antibody 28568-1-AP | Download protocol |
| WB protocol for CENPF antibody 28568-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
28568-1-AP – CENPF Polyclonal Antibody – Citation Summary
This polyclonal antibody has 5 total citations. It appears in the journals Nature Communications, Cancer Cell International, Oncology Letters, Translational Andrology and Urology, and Biochemical Pharmacology. The research fields span cancer biology and oncology, including pancreatic ductal adenocarcinoma (RNA methylation/m6A), glioma growth signaling (mTORC1/NF-κB), lung adenocarcinoma (prognosis, proliferation/migration), renal cell carcinoma proliferation, and glioblastoma drug resistance/epigenetics. Applications cited include Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes | ||
Cancer Cell Int CENPF interaction with PLA2G4A promotes glioma growth by modulating mTORC1 and NF-κB pathways | ||
Oncol Lett FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma | ||
Transl Androl Urol CENPF promotes the proliferation of renal cell carcinoma in vitro
| ||
Biochem Pharmacol KDM1A/HDAC2-driven epigenetic dysregulation maintains a drug-resistant, relapse-initiating glioblastoma cell niche at the peri-tumoral margin. |
