CENPJ Polyclonal antibody, PBS Only

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  WB analysis of mouse testis using 11517-1-AP (same clone as 11517-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11517-1-AP (same clone as 11517-1-PBS)

  WB result of CENPJ antibody (11517-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPJ transfected Jurkat cells. This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11517-1-AP (same clone as 11517-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CENPJ antibody (11517-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11517-1-AP (same clone as 11517-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. . This data was developed using the same antibody clone with 11517-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CENPJ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 11517-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

11517-1-PBS targets CENPJ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2078

  Product name: Recombinant human CENPJ protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1033-1338 aa of BC024209

  Sequence: RENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL

• Full Name: centromere protein J
• Calculated Molecular Weight: 1338 aa, 153 kDa
• Observed Molecular Weight: 153 kDa
• GenBank Accession Number: BC024209
• Gene Symbol: CENPJ
• Gene ID (NCBI): 55835
• RRID: AB_2244605
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC77
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.