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CENPJ Polyclonal antibody

CENPJ Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11517-1-AP

Synonyms

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAP, LIP1, LYST interacting protein 1, MCPH6



Tested Applications

Positive WB detected inmouse testis tissue, Jurkat cells
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11517-1-AP targets CENPJ in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CENPJ fusion protein Ag2078
Full Name centromere protein J
Calculated Molecular Weight 1338 aa, 153 kDa
Observed Molecular Weight 153 kDa
GenBank Accession NumberBC024209
Gene Symbol CENPJ
Gene ID (NCBI) 55835
RRIDAB_2244605
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Protocols

Product Specific Protocols
WB protocol for CENPJ antibody 11517-1-APDownload protocol
IF protocol for CENPJ antibody 11517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.

Authors - Franz Meitinger
humanIF

Science

Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.

Authors - Yao Liang Wong
mouseWB,IF

Nat Cell Biol

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Authors - Huijie Zhao
mouseWB,IF

Neuron

Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.

Authors - Divya Jayaraman
ratIF

Dev Cell

SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication.

Authors - Chii Shyang Fong
humanIF

Nat Commun

Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.

Authors - Søs Grønbæk Holdgaard