CEP135 Polyclonal antibody

CEP135 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG


human, Canine and More (1)





Cat no : 24428-1-AP


centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, U2OS cells, U-87 MG cells
Positive IF detected inMDCK cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP135 fusion protein Ag19122
Full Name centrosomal protein 135kDa
Calculated Molecular Weight 1140 aa, 134 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC136535
Gene Symbol CEP135
Gene ID (NCBI) 9662
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP135 is a 135 kDa centrosomal protein that is present in a wide range of organisms. CEP135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles.


Product Specific Protocols
WB protocol for CEP135 antibody 24428-1-APDownload protocol
IF protocol for CEP135 antibody 24428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Curr Biol

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Authors - Hindol Gupta


ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Authors - Ting Song


WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.

Authors - Emmanuelle Steib


Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.

Authors - Zhenzhen Chu

Mol Biol Cell

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.

Authors - Alexander J Stemm-Wolf


A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).

Authors - Yan-Wei Sha


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Elisa (Verified Customer) (01-25-2023)

RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
CEP135 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24428-1-AP)