CEP135 Polyclonal antibody
CEP135 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine and More (1)
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 24428-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, K-562 cells, U2OS cells, U-87 MG cells |
Positive IF detected in | MDCK cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
IF | See 5 publications below |
Product Information
24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.
Tested Reactivity | human, Canine |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CEP135 fusion protein Ag19122 |
Full Name | centrosomal protein 135kDa |
Calculated molecular weight | 1140 aa, 134 kDa |
Observed molecular weight | 133 kDa |
GenBank accession number | BC136535 |
Gene symbol | CEP135 |
Gene ID (NCBI) | 9662 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for CEP135 antibody 24428-1-AP | Download protocol |
IF protocol for CEP135 antibody 24428-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Curr Biol SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells. | ||
EMBO Rep ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. | ||
Elife WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity. | ||
Cells Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells. | ||
Mol Biol Cell The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis. | ||
Gene A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF). |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elisa (Verified Customer) (01-25-2023) | RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
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