|Positive WB detected in||HeLa cells, K-562 cells, U2OS cells, U-87 MG cells|
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.
|Tested Reactivity||human, Canine|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CEP135 fusion protein Ag19122|
|Full Name||centrosomal protein 135kDa|
|Calculated molecular weight||1140 aa, 134 kDa|
|Observed molecular weight||133 kDa|
|GenBank accession number||BC136535|
|Gene ID (NCBI)||9662|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.
ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.
WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.
Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.
Mol Biol Cell
The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.
A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).
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Elisa (Verified Customer) (01-25-2023)
RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).