CEP135 Polyclonal antibody

CEP135 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24428-1-AP

Synonyms

centrosomal protein 135kDa, Centrosomal protein 4, Centrosomal protein of 135 kDa, CEP135, CEP4, KIAA0635

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Validation Data Gallery

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  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 24428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24428-1-AP (CEP135 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MDCK using 24428-1-AP

  Immunofluorescent analysis of MDCK cells using 24428-1-AP (CEP135 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, U2OS cells, U-87 MG cells
• Positive IF detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 5 publications below

Product Information

24428-1-AP targets CEP135 in WB, IF, ELISA applications and shows reactivity with human, Canine samples.

• Tested Reactivity: human, Canine
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CEP135 fusion protein Ag19122
• Full Name: centrosomal protein 135kDa
• Calculated molecular weight: 1140 aa, 134 kDa
• Observed molecular weight: 133 kDa
• GenBank accession number: BC136535
• Gene symbol: CEP135
• Gene ID (NCBI): 9662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human		Elife; WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.; Authors - Emmanuelle Steib
human	IF	Cells; Mitotic Maturation Compensates for Premature Centrosome Splitting and PCM Loss in Human cep135 Knockout Cells.; Authors - Zhenzhen Chu
human	IF	Mol Biol Cell; The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.; Authors - Alexander J Stemm-Wolf
human	IF	Gene; A homozygous CEP135 mutation is associated with multiple morphological abnormalities of the sperm flagella (MMAF).; Authors - Yan-Wei Sha

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elisa (Verified Customer) (01-25-2023)

RPE1 cells stained for Hoechst (DNA marker, in blue), Cep135 (in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)

CEP135 Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24428-1-AP)