Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27325-1-AP targets CEP170 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26311 Product name: Recombinant human CEP170 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1012-1202 aa of BC140794 Sequence: EASDSELADADKASVASEVSTTSSTSKPPTGRRNISRIDLLAQPRRTRLGSLSARSDSEATISRSSASSRTAEAIIRSGARLVPSDKFSPRIRANSISRLSDSKVKSMTSAHGSASVNSRWRRFPTDYASTSEDEFGSNRNSPKHTRLRTSPALKTTRLQSAGSAMPTSSSFKHRIKEQEDYIRDWTAHRE Predict reactive species |
| Full Name | centrosomal protein 170kDa |
| Calculated Molecular Weight | 1486 aa, 165 kDa |
| Observed Molecular Weight | 170-190 kDa |
| GenBank Accession Number | BC140794 |
| Gene Symbol | CEP170 |
| Gene ID (NCBI) | 9859 |
| RRID | AB_2880844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SW79 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP170 antibody 27325-1-AP | Download protocol |
| WB protocol for CEP170 antibody 27325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP170 Polyclonal antibody (Cat# 27325-1-AP) has 11 citations published in journals including Science, Nature Communications, Cell Reports, Journal of Cell Biology, Journal of Cell Science, Cell Communication and Signaling, Frontiers in Immunology, Reproductive Biomedicine Online, eLife, and bioRxiv. Research fields span cell biology (acentrosomal spindle assembly, centrosome/cilia structure, microtubule dynamics), immunology (T-cell architecture, anti-tumor immunity), reproductive biology (spermatogenesis, oocyte development), and cell signaling (DNA damage repair, protein catabolism).
| Species | Application | Title |
|---|---|---|
Science A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. | ||
Cell Commun Signal A microprotein encoded by FERMT3 modulates endothelial cell protein catabolism and induces cell cycle arrest and senescence. | ||
Cell Rep Unveiling the molecular architecture of T cells and immune synapses with cryo-expansion microscopy. | ||
Front Immunol Hypoxia reconstructed colorectal tumor microenvironment weakening anti-tumor immunity: construction of a new prognosis predicting model through transcriptome analysis |
Reviews
What customers say
Both reviewers (5/5) used this CEP170 antibody for Western Blot, with one also applying it for Immunofluorescence. The WB protocol was 1:1000 in 5% milk TBS overnight (LI-COR), noting the observed molecular weight is higher than calculated. The IF dilution was 1/200 on RPE1 cells. One reviewer specifically compared it to antibodies from other companies and stated this one performed the best for both IF and WB applications. Overall, users report strong, reliable performance across tested applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ilham (Verified Customer) (08-06-2024) | CEP170 works very well I confirm that the observed molecular weight is higher than the calculated molecular weight, using at 1:1000 in 5% milk TBSt O/N (LI-COR).
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Laura (Verified Customer) (02-27-2019) | Comparing with antibodies from other companies that we bought, this antibody is the best that we tried for IF and Western Blot !
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