Centrin 3 Polyclonal antibody

Centrin 3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15811-1-AP

Synonyms

CEN3, Centrin 3, CETN3

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Validation Data Gallery

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  WB analysis using 15811-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15811-1-AP (Centrin 3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 15811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: NCI-H1299 cells, C2C12 cells, Neuro-2a cells, mouse ovary tissue, mouse testis tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15811-1-AP targets Centrin 3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Centrin 3 fusion protein Ag8547
• Full Name: centrin, EF-hand protein, 3 (CDC31 homolog, yeast)
• Calculated Molecular Weight: 167 aa, 20 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC005383
• Gene Symbol: CETN3
• Gene ID (NCBI): 1070
• RRID: AB_2082361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; Zika virus alters centrosome organization to suppress the innate immune response.; Authors - Andrew Kodani