CFHR3 Polyclonal antibody

CFHR3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16583-1-AP

Synonyms

CFHL3, CFHR3, complement factor H related 3, DOWN16, FHR 3, FHR3, H factor like protein 3, HLF4



Tested Applications

Positive WB detected inhuman heart tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16583-1-AP targets CFHR3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFHR3 fusion protein Ag9963
Full Name complement factor H-related 3
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 45-56 kDa
GenBank accession numberBC058009
Gene symbol CFHR3
Gene ID (NCBI) 10878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. A frequent deletion of CFHR1 and CFHR3 genes was found to be associated with decrease risk of aged-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

Protocols

Product Specific Protocols
WB protocol for CFHR3 antibody 16583-1-APDownload protocol
IHC protocol for CFHR3 antibody 16583-1-APDownload protocol
IP protocol for CFHR3 antibody 16583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Eye Res

Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.

Authors - Swati Arya
humanWB

Mol Med Rep

Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.

Authors - Hong Liu
humanWB

PLoS One

A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.

Authors - Yoko Yoshida
humanWB

Sci Rep

A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.

Authors - Yin Ding
humanWB

Clin Cancer Res

Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.

Authors - Laura M Rogers