CFHR3 Polyclonal antibody

CFHR3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16583-1-AP

Synonyms

CFHL3, CFHR3, complement factor H related 3, DOWN16, FHR 3, FHR3, H factor like protein 3, HLF4



Tested Applications

Positive WB detected inmouse liver tissue, human heart tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16583-1-AP targets CFHR3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFHR3 fusion protein Ag9963
Full Name complement factor H-related 3
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight 45-56 kDa
GenBank accession numberBC058009
Gene symbol CFHR3
Gene ID (NCBI) 10878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

Protocols

Product Specific Protocols
WB protocol for CFHR3 antibody 16583-1-APDownload protocol
IHC protocol for CFHR3 antibody 16583-1-APDownload protocol
IP protocol for CFHR3 antibody 16583-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Exp Eye Res

Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.

Authors - Swati Arya
humanWB

Mol Med Rep

Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.

Authors - Hong Liu
humanWB

PLoS One

A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.

Authors - Yoko Yoshida
humanWB

Sci Rep

A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.

Authors - Yin Ding
humanWB

Clin Cancer Res

Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.

Authors - Laura M Rogers