|Positive WB detected in||human heart tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16583-1-AP targets CFHR3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CFHR3 fusion protein Ag9963|
|Full Name||complement factor H-related 3|
|Calculated molecular weight||330 aa, 37 kDa|
|Observed molecular weight||45-56 kDa|
|GenBank accession number||BC058009|
|Gene ID (NCBI)||10878|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. A frequent deletion of CFHR1 and CFHR3 genes was found to be associated with decrease risk of aged-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).
Exp Eye Res
Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.
Mol Med Rep
Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.
A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.
A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.
Clin Cancer Res
Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.