Chromogranin A Polyclonal antibody

Chromogranin A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10529-1-AP

Synonyms

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2



Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inhuman pancreas tissue, human colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

10529-1-AP targets Chromogranin A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chromogranin A fusion protein Ag0807
Full Name chromogranin A (parathyroid secretory protein 1)
Calculated molecular weight 51 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC006459
Gene symbol CHGA
Gene ID (NCBI) 1113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Protocols

Product Specific Protocols
WB protocol for Chromogranin A antibody 10529-1-APDownload protocol
IHC protocol for Chromogranin A antibody 10529-1-APDownload protocol
IF protocol for Chromogranin A antibody 10529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Rep

Protein O-GlcNAc Modification Links Dietary and Gut Microbial Cues to the Differentiation of Enteroendocrine L Cells.

Authors - Ming Zhao
humanWB

Sci Rep

Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line.

Authors - Azita Monazzam

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (08-14-2020)

Used 1:1000 dilution for immunoblotting. It gives a very clean band.

  • Applications: Western Blot,Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer