|Positive WB detected in||A549 cells, HeLa cells, PC-3 cells, A431 cells, T-47D cells|
|Positive IHC detected in||human lung cancer tissue, human pancreas tissue, human breast cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:10000-1:40000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66483-1-Ig targets Cytokeratin 7 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytokeratin 7 fusion protein Ag7895|
|Full Name||keratin 7|
|Calculated molecular weight||469 aa, 51 kDa|
|Observed molecular weight||51 kDa|
|GenBank accession number||BC002700|
|Gene ID (NCBI)||3855|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7, also named as cytokeratin 7, is one member of type II basic cytokeratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels, and their neoplasms. KRT7 is marker of epithelial tissues, but not present in carcinomas of stratified squamous cell origin.This antibody is specifically against KRT7.
Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.
Cyclophilin A inhibits trophoblast migration and invasion in vitro and vivo through p38/ERK/JNK pathways and causes features of preeclampsia in mice.
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Santhosh (Verified Customer) (04-15-2019)
Human breast tumor FFPE tissue. HIER using citrate buffer. 1:200