CLCC1 Monoclonal antibody

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  WB analysis of hTERT-RPE1 using 68853-7-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 68853-7-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68853-7-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68853-7-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CLCC1 Monoclonal Antibody for WB, ELISA

Cat No. 68853-7-Ig

Clone No.3C4B4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

Mid-1-related chloride channel protein 1, MCLC, KIAA0761, Chloride channel CLIC-like protein 1, chloride channel CLIC like 1

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells, hTERT-RPE1 cells, HeLa cells, K-562 cells, FaDu cells, SiHa cells, SCaBER cells, MG-63 cells, MDA-MB-468 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68853-7-Ig targets CLCC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24682

  Product name: Recombinant human CLCC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 315-501 aa of BC002939

  Sequence: GPESEPPQALRPRDRRRQEEIDYRPDGGAGDADFHYRGQMGPTEQGPYAKTYEGRREILRERDVDLRFQTGNKSPEVLRAFDVPDAEAREHPTVVPSHKSPVLDTKPKETGGILGEGTPKESSTESSQSAKPVSGQDTSGNTEGSPAAEKAQLKSEAAGSPDQGSTYSPARGVAGPRGQDPVSSPCG

• Full Name: chloride channel CLIC-like 1
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC002939
• Gene Symbol: CLCC1
• Gene ID (NCBI): 23155
• RRID: AB_3670441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q96S66
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLCC1, also known as MCLC, is a transmembrane protein that localizes to intracellular compartments, including endoplasmic reticulum (ER), and it functions as a chloride channel (PMID: 11279057). It has been shown that loss of CLCC1 results in ER Stress, misfolded protein accumulation, and neurodegeneration (PMID: 25698737). CLCC1 forms homomultimer in the ER membrane and is a pore-forming component of an anion channel. Studies found that disruption of ER ion homeostasis maintained by CLCC1 is one of the causes of neurodegenerative diseases, such as ALS (amyotrophic lateral sclerosis) (PMID: 37142673).