Claudin 18 Polyclonal antibody

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  WB analysis of mouse kidney using 21126-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21126-1-AP (Claudin 18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 21126-1-AP

  IP result of anti-Claudin 18 (IP:21126-1-AP, 3ug; Detection:21126-1-AP 1:500) with mouse stomach tissue lysate 4000ug.

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MKN-45 using 21126-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MKN-45 cells using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Claudin 18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21126-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

UNQ778/PRO1572, CLDN18, Claudin-18, Claudin18

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IP detected in: mouse stomach tissue
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue, human ovary tumor tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MKN-45 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Product Information

21126-1-AP targets Claudin 18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 18 fusion protein Ag15517
• Full Name: claudin 18
• Calculated Molecular Weight: 261 aa, 28 kDa
• Observed Molecular Weight: 28-29 kDa
• GenBank Accession Number: BC146668
• Gene Symbol: CLDN18
• Gene ID (NCBI): 51208
• RRID: AB_10733638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P56856
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	IHC	Ann Transl Med; Inhibition of protein kinase C alpha attenuates lipopolysaccharide-triggered acute lung injury by alleviating the hyperinflammatory response and oxidative stress.; Authors - Yang Chen
mouse	WB,IF	Int J Mol Med; Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.; Authors - Ruixue Yang
mouse	IF	BMC Pulm Med; SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure.; Authors - Chih-Hao Shen
rat	IF	J Stroke Cerebrovasc Dis; Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.; Authors - Chunyan Wang
human	IHC	Cancers (Basel); Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases; Authors - Maximiliano Gelli