Claudin 18 Polyclonal antibody

Claudin 18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21126-1-AP

Synonyms

claudin 18, CLDN18, SFTA5, SFTPJ



Tested Applications

Positive WB detected inmouse kidney tissue
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human ovary tumor tissue, human pancreas cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMKN-45 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

21126-1-AP targets Claudin 18 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 18 fusion protein Ag15517
Full Name claudin 18
Calculated molecular weight 261 aa, 28 kDa
Observed molecular weight 28-29 kDa
GenBank accession numberBC146668
Gene symbol CLDN18
Gene ID (NCBI) 51208
RRIDAB_10733638
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Protocols

Product Specific Protocols
WB protocol for Claudin 18 antibody 21126-1-APDownload protocol
IHC protocol for Claudin 18 antibody 21126-1-APDownload protocol
IF protocol for Claudin 18 antibody 21126-1-APDownload protocol
IP protocol for Claudin 18 antibody 21126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Authors - Xiaoxue Ren
mouseIHC

Ann Transl Med

Inhibition of protein kinase C alpha attenuates lipopolysaccharide-triggered acute lung injury by alleviating the hyperinflammatory response and oxidative stress.

Authors - Yang Chen
mouseWB,IF

Int J Mol Med

Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.

Authors - Ruixue Yang
mouseIF

BMC Pulm Med

SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure.

Authors - Chih-Hao Shen
ratIF

J Stroke Cerebrovasc Dis

Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.

Authors - Chunyan Wang
humanIHC

Cancers (Basel)

Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases

Authors - Maximiliano Gelli