CLEC14A Polyclonal antibody

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  WB analysis of human heart using 23964-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23964-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23964-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CLEC14A Polyclonal Antibody for WB, ELISA

Cat No. 23964-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, human heart tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

23964-1-AP targets CLEC14A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21081

  Product name: Recombinant human CLEC14A protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-349 aa of BC031567

  Sequence: MRPAFALCLLWQALWPGPGGGEHPTADRAGCSASGACYSLHHATMKRQAAEEACILRGGALSTVRAGAELRAVLALLRAGPGPGGGSKDLLFWVALERRRSHCTLENEPLRGFSWLSSDPGGLESDTLQWVEEPQRSCTARRCAVLQATGGVEPAGWKEMRCHLRANGYLCKYQFEVLCPAPRPGAASNLSYRAPFQLHSAALDFSPPGTEVSALCRGQLPISVTCIADEIGARWDKLSGDVLCPCPGRYLRAGKCAELPNCLDDLGGFACECATGFELGKDGRSCVTSGEGQPTLGGTGVPTRRPPATATSPVPQRTWPIRVDEKLGETPLVPEQDNSVTSIPEIPRW

• Full Name: C-type lectin domain family 14, member A
• Calculated Molecular Weight: 490 aa, 52 kDa
• Observed Molecular Weight: 52 kDa, 100 kDa
• GenBank Accession Number: BC031567
• Gene Symbol: CLEC14A
• Gene ID (NCBI): 161198
• RRID: AB_2879377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q86T13
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	FASEB J; CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.; Authors - Zeyu Su