Calsyntenin-1 Polyclonal antibody

Calsyntenin-1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12788-1-AP

Synonyms

ALC ALPHA, Alcadein alpha, alcalpha1, alcalpha2, C terminal fragment 1 alpha, calsyntenin 1, Calsyntenin-1, CLSTN1, CS1, CSTN1, KIAA0911, PIK3CD, SAlc alpha CTF1 alpha, XB31alpha



Tested Applications

Positive WB detected inhuman brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12788-1-AP targets Calsyntenin-1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calsyntenin-1 fusion protein Ag3504
Full Name calsyntenin 1
Calculated molecular weight 109 kDa
Observed molecular weight 109-130 kDa
GenBank accession numberBC033902
Gene symbol CLSTN1
Gene ID (NCBI) 22883
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).

Protocols

Product Specific Protocols
WB protocol for Calsyntenin-1 antibody 12788-1-APDownload protocol
IHC protocol for Calsyntenin-1 antibody 12788-1-APDownload protocol
IP protocol for Calsyntenin-1 antibody 12788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.

Authors - Yi-Yuan Sun
mouseIF

J Neurosci

The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites.

Authors - Muresan Virgil V

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (12-09-2019)

This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.

  • Applications: Western Blot,