Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12788-1-AP targets Calsyntenin-1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3504 Product name: Recombinant human CLSTN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-350 aa of BC033902 Sequence: ARVNKHKPWLEPTYHGIVTENDNTVLLDPPLIALDKDAPLRFAGEICGFKIHGQNVPFDAVVVDKSTGEGVIRSKEKLDCELQKDYSFTIQAYDCGKGPDGTNVKKSHKATVHIQVNDVNEYAPVFKEKSYKATVIEGKQYDSILRVEAVDADCSPQFSQICSYEIITPDVPFTVDKDGYIKNTEKLNYGKEHQYKLTVTAYDCGKKRATEDVLVKISIKPTCTPGWQGWNNRIEYEPGTGALAVFPNIHLETCDEPVASVQATVELETSHIGKGCDRDTYSEKSLHRLCGAAAGTAELLPSPSGSLNWTMGLPTDNGHDSD Predict reactive species |
| Full Name | calsyntenin 1 |
| Calculated Molecular Weight | 109 kDa |
| Observed Molecular Weight | 109-130 kDa |
| GenBank Accession Number | BC033902 |
| Gene Symbol | Calsyntenin-1 |
| Gene ID (NCBI) | 22883 |
| RRID | AB_2229723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94985 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| IP protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| WB protocol for Calsyntenin-1 antibody 12788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Calsyntenin-1 Polyclonal antibody (Cat# 12788-1-AP) has 9 citations published in journals including Cell, Oncogene, Cell Death Discovery, Cell Reports, Journal of Neuroscience, Journal of Inflammation Research, Cardiovascular Drugs and Therapy, Frontiers in Oncology, and Journal of Cancer Research and Clinical Oncology. Research fields span Alzheimer's disease, gastric cancer metastasis, breast cancer EMT, embryonic development, rheumatoid arthritis, neuronal vesicular transport, dilated cardiomyopathy, and endometrial cancer.
| Species | Application | Title |
|---|---|---|
Cell Peripheral cancer attenuates amyloid pathology in Alzheimer's disease via cystatin-c activation of TREM2. | ||
Cell Death Discov ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer | ||
Oncogene TIA1 depletion enhances CLSTN1 exon 11 inclusion to facilitate Epithelial-to-Mesenchymal transition and breast cancer metastasis. | ||
J Inflamm Res Unveiling the Therapeutic Potential of Berberine in Rheumatoid Arthritis: A Comprehensive Study of Network Pharmacology, Metabolomics, and Intestinal Flora | ||
J Neurosci The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites. |
Reviews
What customers say
One reviewer (Boyan Zhang, Dec 2019) rated this antibody 1 out of 5 for Western Blot. They reported a clean band at ~100 kD matching the datasheet, but noted that literature shows Clstn1 typically runs as doublet bands at 130–150 kD due to glycosylation. The reviewer expressed concern that the antibody may recognize a different protein rather than Clstn1 itself.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (12-09-2019) | This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.
|
