Calsyntenin-1 Polyclonal antibody

Calsyntenin-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (1)

Cat no : 12788-1-AP

Synonyms

ALC ALPHA, Alcadein alpha, alcalpha1, alcalpha2, C terminal fragment 1 alpha, calsyntenin 1, Calsyntenin-1, CLSTN1, CS1, CSTN1, KIAA0911, PIK3CD, SAlc alpha CTF1 alpha, XB31alpha

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Validation Data Gallery

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  WB analysis of human brain using 12788-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12788-1-AP

  IP result of anti-Calsyntenin-1 (IP:12788-1-AP, 4ug; Detection:12788-1-AP 1:300) with mouse brain tissue lysate 5600ug.

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  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12788-1-AP (Calsyntenin-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12788-1-AP targets Calsyntenin-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calsyntenin-1 fusion protein Ag3504
• Full Name: calsyntenin 1
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 109-130 kDa
• GenBank Accession Number: BC033902
• Gene Symbol: CLSTN1
• Gene ID (NCBI): 22883
• RRID: AB_2229723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).

Publications

Species	Application	Title
mouse	IF	J Neurosci; The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites.; Authors - Muresan Virgil V
human	WB	Front Oncol; Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.; Authors - Yi-Yuan Sun
rat	WB,IHC	Cardiovasc Drugs Ther; Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats; Authors - Mingxiang Zhu
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	WB	Cell Death Discov; ESRP1-driven alternative splicing of CLSTN1 inhibits the metastasis of gastric cancer; Authors - Chengguo Li
human	WB	J Cancer Res Clin Oncol; Explore the alterations of downstream molecular pathways caused by ARID1A mutation/knockout in human endometrial cancer cells; Authors - Baoling Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (12-09-2019)

This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.

• Applications: Western Blot,