|Positive WB detected in||human brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2400|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12788-1-AP targets Calsyntenin-1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Calsyntenin-1 fusion protein Ag3504|
|Full Name||calsyntenin 1|
|Calculated molecular weight||109 kDa|
|Observed molecular weight||109-130 kDa|
|GenBank accession number||BC033902|
|Gene ID (NCBI)||22883|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Calsyntenins (calsyntenin-1, -2, -3) compose a family of neuronally expressed proteins that belong to the cadherin superfamily of cell adhesion molecules (PMID: 24966372; 12498782). Calsyntenin-1 (CLSTN1) is a proteolytically processed postsynaptic membrane protein with a cytoplasmic calcium-binding domain. By binding Ca2+, calsyntenin-1 may modulate Ca2+-mediated postsynaptic signals (PMID: 11161476). Calsyntenin-1 is a transmembrane cargo-docking protein for Kinesin-1-mediated vesicular transport (PMID: 16760430). It can mediate axonal transport of the amyloid precursor protein and regulate Aβ production (PMID: 22434822).
The cleavage products of amyloid-beta precursor protein are sorted to distinct carrier vesicles that are independently transported within neurites.
Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.
Cardiovasc Drugs Ther
Calsyntenin-1 Promotes Doxorubicin-induced Dilated Cardiomyopathy in Rats
A program of successive gene expression in mouse one-cell embryos
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (12-09-2019)
This antibody gave a clean band around 100 kD, like that is shown in its datasheet. However, in literature, people showed that this protein runs as doublet bands at 130 - 150 kD due to its glycosylation. Therefore, this antibody is highly suspicious for recognizing another protein rather than Clstn1 itself.