CLSTN2 Polyclonal antibody, PBS Only

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  WB analysis using 21436-1-AP (same clone as 21436-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21436-1-AP (CLSTN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21436-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21436-1-AP (same clone as 21436-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21436-1-AP (CLSTN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21436-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 21436-1-AP (same clone as 21436-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CLSTN2 antibody (21436-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21436-1-PBS in a different storage buffer formulation.

CLSTN2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 21436-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ALC GAMMA, Alcadein gamma, Alcadein-gamma, Alc-gamma, calsyntenin 2

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Product Information

21436-1-PBS targets CLSTN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16048

  Product name: Recombinant human CLSTN2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 473-772 aa of BC104485

  Sequence: ATYEPYLVTNDWPIHPSHIAMQLTVGACWQGGEVTKPQFAQFFHGSLASLTIRPGKMESQKVISCLQACKEGLDINSLESLGQGIKYHFNPSQSILVMEGDDIGNINRALQKVSYINSRQFPTAGVRRLKVSSKVQCFGEDVCISIPEVDAYVMVLQAIEPRITLRGTDHFWRPAAQFESARGVTLFPDIKIVSTFAKTEAPGDVKTTDPKSEVLEEMLHNLDFCDILVIGGDLDPRQECLELNHSELHQRHLDATNSTAGYSIYGVGSMSRYEQVLHHIRYRNWRPASLEARRFRIKCS

• Full Name: calsyntenin 2
• Calculated Molecular Weight: 955 aa, 107 kDa
• Observed Molecular Weight: 100-107 kDa
• GenBank Accession Number: BC104485
• Gene Symbol: CLSTN2
• Gene ID (NCBI): 64084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4D0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CLSTN2 gene encodes the synaptic protein calsyntenin 2, one of the three calsyntenin family members found in humans. CLSTN2 is expressed exclusively in the brain, with high levels occurring in cortical gamma-aminobutyric acid (GABA) interneurons and in medial temporal lobe regions (PMID: 19058786). Western blot analysis detected CLSTN2 at an apparent molecular mass of 107 kDa.