UMP/CMP kinase Polyclonal antibody

UMP/CMP kinase Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11360-1-AP

Synonyms

CMPK1, Cytidylate kinase, Cytidine monophosphate kinase, CMPK, CMK

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Validation Data Gallery

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  WB analysis of mouse pancreas using 11360-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human pancreas using 11360-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11360-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11360-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, human pancreas tissue, human skeletal muscle tissue
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11360-1-AP targets UMP/CMP kinase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UMP/CMP kinase fusion protein Ag1920
• Full Name: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC014961
• Gene Symbol: CMPK1
• Gene ID (NCBI): 51727
• RRID: AB_2081395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

Publications

Species	Application	Title
		J Proteomics; Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.; Authors - Víctor Llombart