CNGB1 Polyclonal antibody

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  WB analysis using 30557-1-AP

  Mouse retina tissue lysates were subjected to SDS PAGE followed by western blot with 30557-1-AP (CNGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse eye using 30557-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CNGB1 antibody (30557-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CNGB1 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 30557-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

CNCG2, CNCG3L, CNCG4, CNG 4, CNG channel 4

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Tested Applications

• Positive WB detected in: mouse retina tissue
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30557-1-AP targets CNGB1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22914

  Product name: Recombinant human CNGB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 343-656 aa of BC156722

  Sequence: MPRELSRIEEEKEDEEEEEEEEEEEEEEEVTEVLLDSCVVSQVGVGQSEEDGTRPQSTSDQKLWEEVGEEAKKEAEEKAKEEAEEVAEEEAEKEPQDWAETKEEPEAEAEAASSGVPATKQHPEVQVEDTDADSCPLMAEENPPSTVLPPPSPAKSDTLIVPSSASGTHRKKLPSEDDEAEELKALSPAESPVVAWSDPTTPKDTDGQDRAASTASTNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPDTKPAEAEPVEEEHYCDMLCCKFKHRPWKKYQFPQSIDPLTNLMY

• Full Name: cyclic nucleotide gated channel beta 1
• Calculated Molecular Weight: 1251 aa, 140 kDa
• Observed Molecular Weight: 240 kDa
• GenBank Accession Number: BC156722
• Gene Symbol: CNGB1
• Gene ID (NCBI): 1258
• RRID: AB_3086362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14028
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.