Collagen Type XII Polyclonal antibody

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  WB analysis using 30045-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30045-1-AP (COL12A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 30045-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COL12A1 antibody (30045-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Collagen Type XII Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30045-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

COL12, COL12A1, COL12A1L, Collagen alpha 1(XII) chain, Collagen alpha-1(XII) chain

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, SKOV-3 cells, C2C12 cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30045-1-AP targets Collagen Type XII in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type XII fusion protein Ag32602
• Full Name: collagen, type XII, alpha 1
• Calculated Molecular Weight: 333kd
• Observed Molecular Weight: 310-333 kDa
• GenBank Accession Number: NM_004370
• Gene Symbol: COL12A1
• Gene ID (NCBI): 1303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99715
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL12A1 (Collagen alpha-1(XII) chain), also known as COL12A1L. It is predicted to be located in the ECM. Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. The molecular weight of COL12A1 is 333 kDa.