Collagen Type I Polyclonal antibody

Collagen Type I Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human, pig, rat and More (3)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14695-1-AP

Synonyms

Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4



Tested Applications

Positive WB detected inpig skin tissue, mouse lung tissue, rat lung tissue, human placenta tissue, human kidney tissue
Positive IHC detected inhuman skin cancer tissue, human colon tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

14695-1-AP targets Collagen Type I in WB, IHC, IF,ELISA applications and shows reactivity with mouse, human, pig, rat samples.

Tested Reactivity mouse, human, pig, rat
Cited Reactivity canine, human, mouse, pig, rabbit, rat, toad
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type I fusion protein Ag6281
Full Name collagen, type I, alpha 2
Calculated molecular weight 1366 aa, 130 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC054498
Gene symbol COL1A2
Gene ID (NCBI) 1278
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type I collagen can recognize collagen alpha 2(I) chain and C-terminal propeptide of pro-alpha 2(I) chain.

Protocols

Product Specific Protocols
WB protocol for Collagen Type I antibody 14695-1-APDownload protocol
IHC protocol for Collagen Type I antibody 14695-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,ratWB,IF

FEBS J

Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.

Authors - Yi-Xuan Chen
humanWB,IF

Clin Sci (Lond)

Involvement of serum amyloid A1 in the rupture of fetal membranes through induction of collagen I degradation.

Authors - Wang-Sheng Wang
humanWB

Invest Ophthalmol Vis Sci

A Novel Tissue-Engineered Corneal Stromal Equivalent Based on Amniotic Membrane and Keratocytes.

Authors - Xin Che
mouseWB

ACS Appl Mater Interfaces

Electrophoretic Deposition of Bioadaptive Drug Delivery Coatings on Magnesium Alloy for Bone Repair.

Authors - Hongfei Qi
ratWB

J Cell Physiol

High cholesterol inhibits tendon-related gene expressions in tendon-derived stem cells through reactive oxygen species-activated nuclear factor-κB signaling.

Authors - Kaiqun Li
humanWB

Biomed Pharmacother

Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro.

Authors - Deguang Kong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hala (Verified Customer) (02-23-2021)

very good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
FH

Eistine (Verified Customer) (05-31-2019)

The antibody works pretty good

  • Applications: Immunofluorescence,