Validation Data Gallery
|Positive WB detected in||pig skin tissue, mouse lung tissue, rat lung tissue, human placenta tissue, human kidney tissue|
|Positive IHC detected in||human skin cancer tissue, human colon tissue, human oesophagus cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
14695-1-AP targets Collagen Type I in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with mouse, human, pig, rat samples.
|Tested Reactivity||mouse, human, pig, rat|
|Cited Reactivity||human, mouse, rat, bovine, canine, pig, rabbit, toad|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type I fusion protein Ag6281|
|Full Name||collagen, type I, alpha 2|
|Calculated molecular weight||1366 aa, 130 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC054498|
|Gene ID (NCBI)||1278|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. This antibody raised against 1017-1366 aa of human pro-alpha 2 chain of type I collagen can recognize collagen alpha 2(I) chain and C-terminal propeptide of pro-alpha 2(I) chain.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
PK (Verified Customer) (07-08-2022)
Ren Jie (Verified Customer) (06-22-2022)
Very strong signal for collagen I, needs stringent wash conditions to minimize ladder staining in western blot
S (Verified Customer) (12-01-2021)
Hala (Verified Customer) (02-23-2021)
Eistine (Verified Customer) (05-31-2019)
The antibody works pretty good