Collagen Type III (N-terminal) Polyclonal antibody
Collagen Type III (N-terminal) Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
134
Cat no : 22734-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skin tissue, rat skin tissue |
| Positive IP detected in | mouse skin tissue |
| Positive IHC detected in | human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, mouse colon tissue, mouse kidney tissue, human skin cancer tissue, mouse heart tissue, human colon tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 102 publications below |
| IHC | See 33 publications below |
| IF | See 13 publications below |
Product Information
The immunogen of 22734-1-AP is Collagen Type III (N-terminal) Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | canine, human, mouse, rabbit, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type III (N-terminal) fusion protein Ag18658 |
| Full Name | collagen, type III, alpha 1 |
| Calculated molecular weight | 1466 aa, 139 kDa |
| Observed molecular weight | 140-180 kDa |
| GenBank accession number | BC028178 |
| Gene symbol | COL3A1 |
| Gene ID (NCBI) | 1281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
| IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
| IP protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Oral Rehabil Catabolic changes of rat temporomandibular joint discs induced by unilateral anterior crossbite. | ||
Toxicol Lett The emerging roles of a novel CCCH-type zinc finger protein, ZC3H4, in silica-induced epithelial to mesenchymal transition. | ||
Int J Mol Sci Salidroside Ameliorates Renal Interstitial Fibrosis by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways. | ||
Int J Med Sci Depletion of WNT10A Prevents Tumor Growth by Suppressing Microvessels and Collagen Expression. | ||
Am J Respir Cell Mol Biol Inhibition of Glutaminase 1 Attenuates Experimental Pulmonary Fibrosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryan (Verified Customer) (04-20-2018) | Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections
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