Collagen Type III (N-terminal) Polyclonal antibody

• Featured Product
• KD/KO Validated

Collagen Type III (N-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

242

Reviews (2)

Cat no : 22734-1-AP

Synonyms

COL3A1, Collagen alpha 1(III) chain, Collagen Type III, collagen, type III, alpha 1, EDS4A

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Validation Data Gallery

• 

  WB analysis of mouse skin using 22734-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 22734-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 22734-1-AP

  IP Result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:500) with mouse skin tissue lysate 3200ug.

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, human skin cancer tissue, mouse heart tissue, mouse kidney tissue, human colon tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 180 publications below
• IHC: See 66 publications below
• IF: See 28 publications below

Product Information

The immunogen of 22734-1-AP is Collagen Type III (N-terminal) Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, hamster, pig, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Collagen Type III (N-terminal) fusion protein Ag18658
• Full Name: collagen, type III, alpha 1
• Calculated molecular weight: 1466 aa, 139 kDa
• Observed molecular weight: 140-180 kDa
• GenBank accession number: BC028178
• Gene symbol: COL3A1
• Gene ID (NCBI): 1281
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Publications

Species	Application	Title
mouse,human	WB	Basic Res Cardiol; SAIL: a new conserved anti-fibrotic lncRNA in the heart.; Authors - Shenjian Luo
mouse	WB	J Exp Med; EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.; Authors - Xixi Tao
pig	IF	Biomaterials; A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis.; Authors - Shengyu Wang
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
		Biomaterials; Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.; Authors - Haizhu Kuang
mouse	WB	EMBO J; ER-anchored CRTH2 antagonizes collagen biosynthesis and organ fibrosis via binding LARP6.; Authors - Shengkai Zuo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gayatri (Verified Customer) (06-10-2022)

Good signal but inexplicable molecular weight. Observe bands at 70 kDa instead of expected 140-180 kDa although this product is validated for Rat. Observed in both rat heart and tail lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Heart Tissue

FH

Ryan (Verified Customer) (04-20-2018)

Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: paraffin carotid artery section

Collagen Type III (N-terminal) Antibody Immunofluorescence validation (1:400 dilution) in paraffin carotid artery section (Cat no:22734-1-AP)

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