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Collagen Type III (N-terminal) Polyclonal antibody
Collagen Type III (N-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 22734-1-AP
Validation Data Gallery
|Positive WB detected in||mouse skin tissue, rat skin tissue|
|Positive IP detected in||mouse skin tissue|
|Positive IHC detected in||human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, human skin cancer tissue, mouse heart tissue, mouse kidney tissue, human colon tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human colon tissue|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22734-1-AP is Collagen Type III (N-terminal) Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, canine, hamster, pig, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type III (N-terminal) fusion protein Ag18658|
|Full Name||collagen, type III, alpha 1|
|Calculated molecular weight||1466 aa, 139 kDa|
|Observed molecular weight||140-180 kDa|
|GenBank accession number||BC028178|
|Gene ID (NCBI)||1281|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).
|Product Specific Protocols|
|WB protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|IF protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|IP protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gayatri (Verified Customer) (06-10-2022)
Good signal but inexplicable molecular weight. Observe bands at 70 kDa instead of expected 140-180 kDa although this product is validated for Rat. Observed in both rat heart and tail lysates.
Ryan (Verified Customer) (04-20-2018)
Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections