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Collagen Type III (N-terminal) Polyclonal antibody

Collagen Type III (N-terminal) Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22734-1-AP

Synonyms

COL3A1, Collagen alpha 1(III) chain, Collagen Type III, collagen, type III, alpha 1, EDS4A



Tested Applications

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, human skin cancer tissue, mouse heart tissue, mouse kidney tissue, human colon tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22734-1-AP is Collagen Type III (N-terminal) Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, hamster, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type III (N-terminal) fusion protein Ag18658
Full Name collagen, type III, alpha 1
Calculated molecular weight 1466 aa, 139 kDa
Observed molecular weight 140-180 kDa
GenBank accession numberBC028178
Gene symbol COL3A1
Gene ID (NCBI) 1281
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Protocols

Product Specific Protocols
WB protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IF protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
IP protocol for Collagen Type III (N-terminal) antibody 22734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Basic Res Cardiol

SAIL: a new conserved anti-fibrotic lncRNA in the heart.

Authors - Shenjian Luo
mouseWB

J Exp Med

EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.

Authors - Xixi Tao
pigIF

Biomaterials

A myogenic niche with a proper mechanical stress environment improves abdominal wall muscle repair by modulating immunity and preventing fibrosis.

Authors - Shengyu Wang
mouseIF

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang

Biomaterials

Construction and performance evaluation of Hep/silk-PLCL composite nanofiber small-caliber artificial blood vessel graft.

Authors - Haizhu Kuang
mouseWB

EMBO J

ER-anchored CRTH2 antagonizes collagen biosynthesis and organ fibrosis via binding LARP6.

Authors - Shengkai Zuo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gayatri (Verified Customer) (06-10-2022)

Good signal but inexplicable molecular weight. Observe bands at 70 kDa instead of expected 140-180 kDa although this product is validated for Rat. Observed in both rat heart and tail lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat Heart Tissue
FH

Ryan (Verified Customer) (04-20-2018)

Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: paraffin carotid artery section
Collagen Type III (N-terminal) Antibody Immunofluorescence validation (1:400 dilution) in paraffin carotid artery section (Cat no:22734-1-AP)