Collagen Type III (N-terminal) Polyclonal antibody
Collagen Type III (N-terminal) Polyclonal Antibody for IHC, IP, WB,ELISA
Cat no : 22734-1-AP
|Positive WB detected in||mouse skin tissue, rat skin tissue|
|Positive IP detected in||mouse skin tissue|
|Positive IHC detected in||human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, mouse colon tissue, mouse kidney tissue, human skin cancer tissue, mouse heart tissue, human colon tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22734-1-AP is Collagen Type III (N-terminal) Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type III (N-terminal) fusion protein Ag18658|
|Full Name||collagen, type III, alpha 1|
|Calculated molecular weight||1466 aa, 139 kDa|
|Observed molecular weight||140-180 kDa|
|GenBank accession number||BC028178|
|Gene ID (NCBI)||1281|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).
|Product Specific Protocols|
|WB protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|IP protocol for Collagen Type III (N-terminal) antibody 22734-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ryan (Verified Customer) (04-20-2018)
Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections