Tested Applications
| Positive IF-P detected in | human colon tissue, human tonsillitis tissue, human colon cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-25747 targets CD68 in IF-P applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22815 Product name: Recombinant human CD68 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-319 aa of BC015557 Sequence: SATLLPSFTVTPTVTESTGTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTVHPTSNSTATSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPSPSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPNKTKVQGSCEGAHPHLLLSFPYGHLSFGFMQDLQQKVVYLSYMAVEYNVSFPHAAQWTFSAQNASLRDLQAPLGQSFSCSNSSIILSPAVHLDLLSLRLQAAQLPHTGVFGQSFSCPSDRS Predict reactive species |
| Full Name | CD68 molecule |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC015557 |
| Gene Symbol | CD68 |
| Gene ID (NCBI) | 968 |
| RRID | AB_2919875 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34810 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CD68 antibody CL594-25747 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL594-25747 is a CD68 Polyclonal antibody conjugated with CoraLite® Plus 594. It has 5 total citations published in Nature Immunology, Acta Pharmaceutica Sinica B, Phytomedicine, Frontiers in Molecular Biosciences, and bioRxiv. The associated research fields include breast cancer immunology, idiopathic pulmonary fibrosis, rheumatoid arthritis, spinal cord injury, and cell-based antibody delivery therapy.
| Species | Application | Title |
|---|---|---|
Nat Immunol Chemotherapy-induced CA-repeat DNA fragments in breast cancer trigger antitumor immune responses | ||
Acta Pharm Sin B RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF. | ||
Phytomedicine Acacetin alleviates rheumatoid arthritis by targeting HSP90 ATPase domain to promote COX-2 degradation | ||
Front Mol Biosci Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress. | ||
bioRxiv Once yearly cell-based therapy for sustained and dose tunable delivery of monoclonal antibodies. |
