COL4A6 Polyclonal antibody

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  WB analysis of SGC-7901 using 10807-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10807-1-AP (Collagen Type IV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10807-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10807-1-AP (Collagen Type IV antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

COL4A6 Polyclonal Antibody for ELISA

Cat No. 10807-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Collagen alpha 6(IV) chain, Collagen alpha-6(IV) chain, Collagen Type IV

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Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

10807-1-AP targets COL4A6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1118

  Product name: Recombinant human COL4A6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-73 aa of BC005305

  Sequence: MLINKLWLLLVTLCLTEELAAAGEKSYGKPCGGQDCSGSCQCFPEKGARHNLQLLNDMAGRLYHFSEVLPNLF

• Full Name: collagen, type IV, alpha 6
• Calculated Molecular Weight: 1691 aa, 164 kDa
• GenBank Accession Number: BC005305
• Gene Symbol: COL4A6
• Gene ID (NCBI): 1288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14031
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen alpha-6(IV) chain, known as COL4A6, is a member of the collagen family, the major structural component of basement membranes. There are six type IV collagen isoforms, alpha 1(IV)-alpha 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. COL4A6 forms a triple helical structure with two other alpha-5-chains, which is stabilized by the presence of glycine as every third residue (PMID: 27377778). COL4A6 is located on the X chromosome in a head-to-head manner with COL4A5 closely (PMID: 23714752). COL4A6 is transcribed from two alternative promoters in a tissue-specific fashion. This antibody detected the cleaved fragment of COL4A6 at ~50 kDa (PMID: 7657706)..

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; Hepatitis B virus X protein promotes the development of liver fibrosis and hepatoma through downregulation of miR-30e targeting P4HA2 mRNA.; Authors - G X Feng
human	IF	Polymers (Basel); The Development of a 3D PET Fibrous Scaffold Modified with an Umbilical Cord dECM for Liver Tissue Engineering; Authors - Yang Li
rat	WB	Toxicol Appl Pharmacol; High doses of baicalin induces kidney injury and fibrosis through regulating TGF-β/Smad signaling pathway.; Authors - Yi Cai
human	IHC	J Tissue Eng Regen Med; Topographical and Chemical Inductive Cues Synergistically Enhance the Schwann Cell Differentiation of Aligned Dental Pulp Stem Cell Sheets; Authors - Michelle D Drewry
human	IHC	J Immunol Res; Partial Reconstruction of Uterus Cervix in Rat by Decellularized Human Uterine Cervical Scaffold Combined with Adipose-Derived Stem Cells (ADSCs); Authors - Yuqi Li
mouse	IF	Oral Dis; Artemisinin suppressed tumour growth and induced vascular normalisation in oral squamous cell carcinoma via inhibition of MIF; Authors - Xiang-Hua Yu