COL5A3 Polyclonal antibody

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  WB analysis using 30204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30204-1-AP (COL5A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 30204-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using COL5A3 antibody (30204-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

COL5A3 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30204-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

COL5, Collagen alpha 3(V) chain, Collagen alpha-3(V) chain, Collagen Type V

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293T cells
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30204-1-AP targets COL5A3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30705

  Product name: Recombinant human COL5A3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 238-380 aa of NM_015719

  Sequence: EPETPRPRRKGKGKGRKKGRGRKGKGRKKNKEIWTSSPPPDSAENQTSTDIPKTETPAPNLPPTPTPLVVTSTVTTGLNATILERSLDPDSGTELGTLETKAAREDEEGDDSTMGPDFRAAEYPSRTQFQIFPGAGEKGAKGE

• Full Name: collagen, type V, alpha 3
• Calculated Molecular Weight: 172 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_015719
• Gene Symbol: COL5A3
• Gene ID (NCBI): 50509
• RRID: AB_3669706
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25940
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COL5A3 (Collagen type V alpha 3) belongs to the fibrillar collagen family. Type V collagen (COLV) is a member of a group I collagen (fibrillar forming collagen) and has three varieties of α chains: α1 (COL5A1), α2 (COL5A2), and α3 (COL5A3). COLV is a minor connective tissue component of nearly ubiquitous distribution. It binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This antibody is specific to COL5A3.