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Collagen Type VI Polyclonal antibody

Collagen Type VI Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17023-1-AP

Synonyms

COL6A1, collagen 6A1, Collagen alpha 1(VI) chain, Collagen Type VI, collagen, type VI, alpha 1, OPLL



Tested Applications

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, human liver tissue, mouse liver tissue, human heart tissue, human hepatocirrhosis tissue, human kidney tissue, human placenta tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17023-1-AP targets Collagen Type VI in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type VI fusion protein Ag10288
Full Name collagen, type VI, alpha 1
Calculated molecular weight 1028 aa, 109 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC052575
Gene symbol COL6A1
Gene ID (NCBI) 1291
RRIDAB_2229737
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IHC protocol for Collagen Type VI antibody 17023-1-APDownload protocol
IP protocol for Collagen Type VI antibody 17023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratIF

Cell Death Differ

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Authors - Guangjin Chen
humanIF

Matrix Biol

Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.

Authors - Emna Ouni
humanWB,IHC

Oncogene

Twist1-induced activation of human fibroblasts promotes matrix stiffness by upregulating palladin and collagen α1(VI).

Authors - I García-Palmero
humanWB

Oncotarget

Reactive stroma component COL6A1 is upregulated in castration-resistant prostate cancer and promotes tumor growth.

Authors - Yi-Ping Zhu
humanIHC

Oncotarget

Upregulation of COL6A1 is predictive of poor prognosis in clear cell renal cell carcinoma patients.

Authors - Fangning Wan
mouseWB,IF

Mater Sci Eng C Mater Biol Appl

Coaxial structured drug loaded dressing combined with induced stem cell differentiation for enhanced wound healing.

Authors - Chen Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Malak (Verified Customer) (02-24-2021)

It works well in the western blot

  • Applications: Western Blot