Validation Data Gallery
|Positive WB detected in||mouse heart tissue, human skeletal muscle tissue, human heart tissue, rat heart tissue|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human colon tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue, mouse liver tissue, human lung tissue, human ovary tissue, human hepatocirrhosis tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17023-1-AP targets Collagen Type VI in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type VI fusion protein Ag10288|
|Full Name||collagen, type VI, alpha 1|
|Calculated molecular weight||1028 aa, 109 kDa|
|Observed molecular weight||140 kDa|
|GenBank accession number||BC052575|
|Gene ID (NCBI)||1291|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.
|Product Specific Protocols|
|WB protocol for Collagen Type VI antibody 17023-1-AP||Download protocol|
|IHC protocol for Collagen Type VI antibody 17023-1-AP||Download protocol|
|IP protocol for Collagen Type VI antibody 17023-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.
Exp Cell Res
Proteomic analysis of mouse ovaries during the prepubertal stages.
Methods Mol Biol
Gapmer Antisense Oligonucleotides to Selectively Suppress the Mutant Allele in COL6A Genes in Dominant Ullrich Congenital Muscular Dystrophy.
BMC Res Notes
Authentication of collagen VI antibodies.
Eur J Dermatol
Differential effect of extracellular matrix derived from papillary and reticular fibroblasts on epidermal development in vitro.
Skeletal muscle proteome expression differentiates severity of cancer cachexia in mice and identifies loss of fragile X mental retardation syndrome-related protein 1.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Malak (Verified Customer) (02-24-2021)
It works well in the western blot