Tested Applications
| Positive WB detected in | A431 cells, 143B cells, various samples, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 140 publications below |
| IHC | See 10 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
55070-1-AP targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, monkey, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cytochrome c oxidase II |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 23-26 kDa |
| GenBank Accession Number | YP_003024029 |
| Gene Symbol | MTCO2 |
| Gene ID (NCBI) | 4513 |
| RRID | AB_10859832 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00403 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IF protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IHC protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| IP protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| WB protocol for MTCO2 antibody 55070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis. | ||
Cell Metab Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits. | ||
Cell Metab Transketolase promotes MAFLD by limiting inosine-induced mitochondrial activity | ||
Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. | ||
Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Morgane (Verified Customer) (10-15-2025) | Works best on PVDF, very clear in total protein extract
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FH Matthieu (Verified Customer) (09-24-2025) | Band is clearly visible at the correct size
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FH Manon (Verified Customer) (09-24-2025) | vey nice band
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FH Emilie (Verified Customer) (09-24-2025) | Bands appeared sharp and well defined under the chosen dilution.
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FH Vignesh (Verified Customer) (09-03-2025) | It's a good product with reduced or no nonspecific bands.
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FH Lenie (Verified Customer) (07-18-2025) | The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.
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FH Baptiste (Verified Customer) (05-23-2025) | Working perfectly
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FH Edward (Verified Customer) (11-15-2022) | Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.
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FH Maria (Verified Customer) (03-11-2021) | MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.
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