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  • KD/KO Validated

MTCO2 Polyclonal antibody

MTCO2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 55070-1-AP

Synonyms

COII, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2, mtcox2



Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse liver tissue, mouse brain tissue, human placenta tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse heart tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55070-1-AP targets MTCO2 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome c oxidase II
Calculated molecular weight 26 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberYP_003024029
Gene symbol COX2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

COX2, also named as COII, COXII and MTCO2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to COX2.

Protocols

Product Specific Protocols
WB protocol for MTCO2 antibody 55070-1-APDownload protocol
IHC protocol for MTCO2 antibody 55070-1-APDownload protocol
IF protocol for MTCO2 antibody 55070-1-APDownload protocol
IP protocol for MTCO2 antibody 55070-1-APDownload protocol
FC protocol for MTCO2 antibody 55070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Authors - Yuanyuan Zhao
zebrafishWB

Nucleic Acids Res

Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.

Authors - Danni Chen
mouseWB

Autophagy

Deficiency of mitophagy receptor FUNDC1 impairs mitochondrial quality and aggravates dietary-induced obesity and metabolic syndrome.

Authors - Hao Wu
humanWB

Cell Rep

The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.

Authors - Kerong Wang

Biochim Biophys Acta

Quantitative proteomics in Friedreich's ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis.

Authors - Lorène Télot
humanWB

Cell Metab

Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.

Authors - Ruchi Masand

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)