- Featured Product
- KD/KO Validated
MTCO2 Polyclonal antibody
MTCO2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 55070-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, various samples, mouse heart tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat heart tissue, mouse liver heart tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||A431 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:4000-1:16000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55070-1-AP targets MTCO2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, zebrafish, hamster|
|Host / Isotype||Rabbit / IgG|
|Full Name||cytochrome c oxidase II|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||23-26 kDa|
|GenBank accession number||YP_003024029|
|Gene ID (NCBI)||4513|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.
|Product Specific Protocols|
|WB protocol for MTCO2 antibody 55070-1-AP||Download protocol|
|IHC protocol for MTCO2 antibody 55070-1-AP||Download protocol|
|IF protocol for MTCO2 antibody 55070-1-AP||Download protocol|
|IP protocol for MTCO2 antibody 55070-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.
Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.
Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.
Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.
Active RNA interference in mitochondria.
Base-Resolution Mapping Reveals Distinct m1A Methylome in Nuclear- and Mitochondrial-Encoded Transcripts.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Edward (Verified Customer) (11-15-2022)
Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.
Maria (Verified Customer) (03-11-2021)
MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.