MTCO2 Polyclonal antibody

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• MTCO2 Polyclonal antibody (55070-1-AP)

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MTCO2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 55070-1-AP

Publications(215)

Reviews (9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

COX2, COII, COX 2, COXII, MT CO2

• 

  WB analysis using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55070-1-AP (MTCO2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of 143B.TK.P0 using 55070-1-AP

  WB result of MTCO2 antibody (MTCO2; 1:2000; incubated at room temperature for 1.5 hours) with 143B and 143B.TK.ρ0 cell. 143B is wild-type cell line, 143B.TK.ρ0 is mtDNA deletion cell line.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of various samples using 55070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with PK30006 (OXPHOS Cocktail (Human recommended)) at dilution of 1:4000 incubated at room temperature for 1 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 55070-1-AP

  IP result of anti-MTCO2 (IP:55070-1-AP, 4ug; Detection:55070-1-AP 1:1000) with HeLa cells lysate 1880 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 55070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55070-1-AP (MTCO2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 55070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (55070-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of A431 using 55070-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MTCO2 (55070-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, 143B cells, various samples, HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55070-1-AP targets MTCO2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cytochrome c oxidase II
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 23-26 kDa
• GenBank Accession Number: YP_003024029
• Gene Symbol: MTCO2
• Gene ID (NCBI): 4513
• RRID: AB_10859832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P00403
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Signal Transduct Target Ther; Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes; Authors - Wenyuan Wu
human	WB	Cell; Engineering mtDNA deletions by reconstituting end joining in human mitochondria; Authors - Yi Fu
mouse	IF	Cell; Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.; Authors - Paul K Ziegler
human	WB,IHC	Cell; PAX translocations remodel mitochondrial metabolism through altered leucine usage in rhabdomyosarcoma; Authors - Bhargab Kalita
human	WB	Cell; Dynamic mapping of proteome trafficking within and between living cells by TransitID; Authors - Wei Qin
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang

Reviews

4.4 out of 5

9 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Morgane (Verified Customer) (10-15-2025)

Works best on PVDF, very clear in total protein extract

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

MTCO2 Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:55070-1-AP)

Matthieu (Verified Customer) (09-24-2025)

Band is clearly visible at the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human fibroblasts

Manon (Verified Customer) (09-24-2025)

vey nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Fibroblasts

Emilie (Verified Customer) (09-24-2025)

Bands appeared sharp and well defined under the chosen dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

Vignesh (Verified Customer) (09-03-2025)

It's a good product with reduced or no nonspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293

Lenie (Verified Customer) (07-18-2025)

The IF staining and WB with the followed protocol of ProteinTech did not work on zebrafish tissue, even it describes on the site it should. Is there a possibility to send a working protocol about this Abies for zebrafish? Thanks. The bond which shows is a human patient sample.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/2000 (WB) and 1/200 (IF)
• Cell Tissue Type: Zebrafish

MTCO2 Antibody Western Blot,Immunofluorescence validation (1/2000 (WB) and 1/200 (IF) dilution) in Zebrafish (Cat no:55070-1-AP)

Baptiste (Verified Customer) (05-23-2025)

Working perfectly

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Lymphoblastoid

MTCO2 Antibody Western Blot validation (1:10000 dilution) in Lymphoblastoid (Cat no:55070-1-AP)

Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562 and KCL22

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)