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MTCO2 Polyclonal antibody

MTCO2 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (5)





Cat no : 55070-1-AP


COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2, mtcox2

Tested Applications

Positive WB detected inA431 cells, various samples, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55070-1-AP targets MTCO2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, monkey, zebrafish, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome c oxidase II
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberYP_003024029
Gene Symbol COX2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D). The antibody is specific to MTCO2.


Product Specific Protocols
WB protocol for MTCO2 antibody 55070-1-APDownload protocol
IHC protocol for MTCO2 antibody 55070-1-APDownload protocol
IF protocol for MTCO2 antibody 55070-1-APDownload protocol
IP protocol for MTCO2 antibody 55070-1-APDownload protocol
FC protocol for MTCO2 antibody 55070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Edward (Verified Customer) (11-15-2022)

Used with 12 ug protein lysate MTCO2 works quite well, both with ECL detection and fluorescent secondary antibodies. Sometimes a bit fainter with ECL.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562 and KCL22

Maria (Verified Customer) (03-11-2021)

MTCO2 in treated Human Primary Fibroblasts. 10 ug of total protein. 12% gel. 1:1000 primary ab in BSA 3% in PBST O/N 4ºC incubation. Secondary ab HRP 1:5000 1h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human Primary Fibroblasts
MTCO2 Antibody Western Blot validation (1:1000 dilution) in Human Primary Fibroblasts (Cat no:55070-1-AP)