MTCO3 Recombinant antibody

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  WB analysis using 84197-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84197-1-RR (MTCO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

MTCO3 Recombinant Antibody for WB, ELISA

Cat No. 84197-1-RR

Clone No.241026E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

COX3, MT CO3, COXIII, COX 3, COIII

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84197-1-RR targets MTCO3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome c oxidase III
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: YP_003024032
• Gene Symbol: MTCO3
• Gene ID (NCBI): 4514
• RRID: AB_3671750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P00414
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).