Filter: AllWBIHCIF/ICC

WB analysis using 13274-1-AP (same clone as 13274-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13274-1-AP (CPEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13274-1-AP (same clone as 13274-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (13274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

CPEB1 Polyclonal antibody, PBS Only

CPEB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 13274-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13274-1-PBS targets CPEB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3994 Product name: Recombinant human CPEB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-486 aa of BC035348 Sequence: WDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS Predict reactive species
Full Name	cytoplasmic polyadenylation element binding protein 1
Calculated Molecular Weight	486 aa, 54 kDa
Observed Molecular Weight	60-70kDa
GenBank Accession Number	BC035348
Gene Symbol	CPEB1
Gene ID (NCBI)	64506
RRID	AB_2083771
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BZB8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1.