CPSF7 Recombinant antibody

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  WB analysis using 85203-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85203-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CPSF7 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85203-1-RR

Clone No.242644E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, mouse uterus tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85203-1-RR targets CPSF7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: pre-mRNA cleavage factor I, 59 kDa subunit
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: NM_001136040
• Gene Symbol: CPSF7
• Gene ID (NCBI): 79869
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N684
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.