CRABP1 Polyclonal antibody

CRABP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12588-1-AP

Synonyms

CRABP, CRABP I, CRABP1, CRABPI, RBP5



Tested Applications

Positive WB detected inmouse embryo tissue, human spleen tissue, Transfected HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

12588-1-AP targets CRABP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRABP1 fusion protein Ag3291
Full Name cellular retinoic acid binding protein 1
Calculated molecular weight 137 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC022069
Gene symbol CRABP1
Gene ID (NCBI) 1381
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.

Protocols

Product Specific Protocols
WB protocol for CRABP1 antibody 12588-1-APDownload protocol
IHC protocol for CRABP1 antibody 12588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIF

Elife

Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu
humanWB,IHC

Mol Oncol

CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.

Authors - Fu Yuan-Shan YS
humanWB

Cell Rep

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Authors - Marie Kubota
mouseWB

Virol J

All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice

Authors - Xiaofei Niu

Cell Rep

Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

Authors - Lixiang Sun