Tested Applications
| Positive WB detected in | mouse embryo tissue, human spleen tissue, Transfected HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12588-1-AP targets CRABP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3291 Product name: Recombinant human CRABP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-137 aa of BC022069 Sequence: MPNFAGTWKMRSSENFDELLKALGVNAMLRKVAVAAASKPHVEIRQDGDQFYIKTSTTVRTTEINFKVGEGFEEETVDGRKCRSLATWENENKIHCTQTLLEGDGPKTYWTSELANDELILTFGADDVVCTRIYVRE Predict reactive species |
| Full Name | cellular retinoic acid binding protein 1 |
| Calculated Molecular Weight | 137 aa, 15 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC022069 |
| Gene Symbol | CRABP1 |
| Gene ID (NCBI) | 1381 |
| RRID | AB_2292271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29762 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CRABP1 antibody 12588-1-AP | Download protocol |
| WB protocol for CRABP1 antibody 12588-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRABP1 Polyclonal antibody (Cat# 12588-1-AP) has 12 citations across journals including Science, Nature Communications, Cell Reports, iScience, Molecular Oncology, Virology Journal, Biology of Reproduction, eLife, medRxiv, and bioRxiv. Research fields span neuroscience (brain development, cochlear and hair-cell development, ALS), energy homeostasis, skin development and wound regeneration, oncology (medulloblastoma, breast cancer), virology (influenza), and reproductive biology (rabbit litter size). Applications include IF, WB, and IHC in human, mouse, rabbit, and chicken samples.
| Species | Application | Title |
|---|---|---|
Science Subventricular zone radial glial cells maintain inhibitory neuron production in the human brain. | ||
Nat Commun The ARCCRABP1 neurons play a crucial role in the regulation of energy homeostasis | ||
Cell Rep Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea. | ||
Cell Rep Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration | ||
Mol Oncol CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells. |
