|Positive WB detected in||mouse embryo tissue, human spleen tissue, Transfected HEK-293 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
12588-1-AP targets CRABP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CRABP1 fusion protein Ag3291|
|Full Name||cellular retinoic acid binding protein 1|
|Calculated molecular weight||137 aa, 15 kDa|
|Observed molecular weight||15 kDa|
|GenBank accession number||BC022069|
|Gene ID (NCBI)||1381|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.
Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.
CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.
Single-cell proteomics reveals changes in expression during hair-cell development.