- Featured Product
- KD/KO Validated
CREG1 Polyclonal antibody
CREG1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA, IF
Conjugate
Unconjugated
Cat no : 12220-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, mouse spleen tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
12220-1-AP targets CREG1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CREG1 fusion protein Ag2901 |
Full Name | cellular repressor of E1A-stimulated genes 1 |
Calculated Molecular Weight | 220 aa, 24 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | BC008628 |
Gene Symbol | CREG1 |
Gene ID (NCBI) | 8804 |
RRID | AB_2084095 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).
Protocols
Product Specific Protocols | |
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WB protocol for CREG1 antibody 12220-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses. | ||
Int J Mol Sci TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle. | ||
Carcinogenesis PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1 | ||
J Cell Mol Med Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats. | ||
J Biochem CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.
| ||
FASEB J CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice. |