CREG1 Polyclonal antibody

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• KD/KO Validated

CREG1 Polyclonal Antibody for WB, ELISA

Cat No. 12220-1-AP Copied!

Publications (10)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

CREG, CREG1, Protein CREG1

• 

  WB analysis using 12220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 12220-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12220-1-AP targets CREG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2901

  Product name: Recombinant human CREG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-220 aa of BC008628

  Sequence: MAGLSRGSARALLAALLASTLLALLVSPARGRGGRDHGDWDEASRLPPLPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLSPLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ

• Full Name: cellular repressor of E1A-stimulated genes 1
• Calculated Molecular Weight: 220 aa, 24 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC008628
• Gene Symbol: CREG1
• Gene ID (NCBI): 8804
• RRID: AB_2084095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75629
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).

Publications

What published studies show

The anti-CREG1 antibody (Cat# 12220-1-AP) has 10 total citations across journals including Nature, Advanced Science, FASEB Journal, Cell Signaling, International Immunopharmacology, Carcinogenesis, and others. Research fields span bone biology and osteoporosis, metabolic regulation (obesity, insulin resistance), viral signaling, placental exosome transport, skeletal muscle adaptation, and renal cell carcinoma progression. Applications cited include WB, IF, and IHC in human, mouse, rat, and bovine species.

Automatically generated from the 10 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
human	IF	Adv Sci (Weinh); IL-33-Induced TREM2+ Macrophages Promote Pathological New Bone Formation Through CREG1-IGF2R Axis in Ankylosing Spondylitis; Authors - Wenjun Hao
bovine	WB,IF	Int J Biol Macromol; CREG1 promotes bovine placental trophoblast cells exosome release by targeting IGF2R and participates in regulating organoid differentiation via exosomes transport; Authors - Naihan Yuan ; KD Validated
rat	IHC	Int Immunopharmacol; CREG1 alleviates bone loss in osteoporosis by enhancing the osteogenic differentiation of BMSCs through mitophagy; Authors - Shiyu Sha
mouse	WB	Int J Mol Sci; TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle.; Authors - Haruna Fujiyoshi
		Cell Signal; CREG1 promotes bone formation via targeting RAB7 to activate autophagy in osteoporosis.; Authors - Xiaofeng Li