CREG1 Polyclonal antibody

• 

  WB analysis using 12220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12220-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12220-1-AP (CREG1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

CREG1 Polyclonal Antibody for WB, ELISA

Cat No. 12220-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

CREG, CREG1, Protein CREG1

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

12220-1-AP targets CREG1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2901

  Product name: Recombinant human CREG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-220 aa of BC008628

  Sequence: MAGLSRGSARALLAALLASTLLALLVSPARGRGGRDHGDWDEASRLPPLPPREDAARVARFVTHVSDWGALATISTLEAVRGRPFADVLSLSDGPPGAGSGVPYFYLSPLQLSVSNLQENPYATLTMTLAQTNFCKKHGFDPQSPLCVHIMLSGTVTKVNETEMDIAKHSLFIRHPEMKTWPSSHNWFFAKLNITNIWVLDYFGGPKIVTPEEYYNVTVQ

• Full Name: cellular repressor of E1A-stimulated genes 1
• Calculated Molecular Weight: 220 aa, 24 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC008628
• Gene Symbol: CREG1
• Gene ID (NCBI): 8804
• RRID: AB_2084095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75629
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.; Authors - Rong Zhang
mouse	WB	Int J Mol Sci; TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle.; Authors - Haruna Fujiyoshi
human	WB	Carcinogenesis; PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1; Authors - Ming Xiong
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang
mouse	WB,IHC	J Biochem; CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.; Authors - Tatsuya Kusudo ; KD Validated
		FASEB J; CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.; Authors - Michihiro Hashimoto