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  • KD/KO Validated

CREG1 Polyclonal antibody

CREG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA, IF

Conjugate

Unconjugated

Cat no : 12220-1-AP

Synonyms

CREG, CREG1, Protein CREG1



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12220-1-AP targets CREG1 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CREG1 fusion protein Ag2901
Full Name cellular repressor of E1A-stimulated genes 1
Calculated Molecular Weight 220 aa, 24 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC008628
Gene Symbol CREG1
Gene ID (NCBI) 8804
RRIDAB_2084095
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REG1 (Cellular repressor of E1A-stimulated genes 1) shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro(PMID:9710587). The monomers of CREG can associate into dimers and the dimerization is observed not only in the crystal but also in solution(PMID:16344469).The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that inhibits cell proliferation and/or enhances differentiation. CREG is widely expressed in adult tissues such as the brain, heart, lungs, liver, intestines and kidneys in mice(PMID:20857207).

Protocols

Product Specific Protocols
WB protocol for CREG1 antibody 12220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.

Authors - Rong Zhang
mouseWB

Int J Mol Sci

TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle.

Authors - Haruna Fujiyoshi
humanWB

Carcinogenesis

PABPN1 Promotes clear cell Renal Cell Carcinoma progression by suppressing the alternative polyadenylation of SGPL1 and CREG1

Authors - Ming Xiong
ratWB

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang
mouseWB,IHC

J Biochem

CREG1 administration stimulates BAT thermogenesis and improves diet-induced obesity in mice.

Authors - Tatsuya Kusudo
  • KD Validated

FASEB J

CREG1 stimulates brown adipocyte formation and ameliorates diet-induced obesity in mice.

Authors - Michihiro Hashimoto