CRHR2 Recombinant antibody

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  WB analysis using 85785-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85785-1-RR (CRHR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85785-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85785-1-RR (CRHR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CRHR2 Recombinant Antibody for WB, ELISA

Cat No. 85785-1-RR

Clone No.250043F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R

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Tested Applications

• Positive WB detected in: HepG2 cells, HL-60 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85785-1-RR targets CRHR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: corticotropin releasing hormone receptor 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_001883
• Gene Symbol: CRHR2
• Gene ID (NCBI): 1395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRHR2 is a receptor for corticotropin-releasing factor (CRF) and belongs to the G-protein coupled receptor family. It shows high affinity for CRF and also binds to related peptides such as urocortin I, II, and III. This receptor is involved in mediating the endocrine, autonomic, and behavioral responses to stress and immune challenges.