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Citrate synthase Polyclonal antibody

Citrate synthase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16131-1-AP

Synonyms

Citrate synthase, CS



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16131-1-AP targets Citrate synthase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Citrate synthase fusion protein Ag9117
Full Name citrate synthase
Calculated molecular weight 466 aa, 52 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC010106
Gene symbol CS
Gene ID (NCBI) 1431
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Protocols

Product Specific Protocols
WB protocol for Citrate synthase antibody 16131-1-APDownload protocol
IHC protocol for Citrate synthase antibody 16131-1-APDownload protocol
IF protocol for Citrate synthase antibody 16131-1-APDownload protocol
IP protocol for Citrate synthase antibody 16131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Photochem Photobiol B

Photobiomodulation enhancement of cell proliferation at 660 nm does not require cytochrome c oxidase.

Authors - Paula L V Lima
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
mouseWB

J Mol Med (Berl)

Exogenous H2S switches cardiac energy substrate metabolism by regulating SIRT3 expression in db/db mice.

Authors - Yu Sun
mouseWB

Oxid Med Cell Longev

Exercise Combined with Rhodiola sacra Supplementation Improves Exercise Capacity and Ameliorates Exhaustive Exercise-Induced Muscle Damage through Enhancement of Mitochondrial Quality Control.

Authors - Yaoshan Dun
mouseWB

Mutat Res

Implications of genotypic differences in the generation of a urinary metabolomics radiation signature.

Authors - Evagelia C Laiakis

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

RAHUL (Verified Customer) (07-03-2019)

Works very good with human cell lysates. 1:1000 dilution in 5% milk; 1 hr incubation at RT.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: DU145 prostate cancer cells