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Citrate synthase Polyclonal antibody

Citrate synthase Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16131-1-AP

Synonyms

Citrate synthase, CS



Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16131-1-AP targets Citrate synthase in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, rabbit, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Citrate synthase fusion protein Ag9117
Full Name citrate synthase
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC010106
Gene Symbol CS
Gene ID (NCBI) 1431
RRIDAB_1640013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

Protocols

Product Specific Protocols
WB protocol for Citrate synthase antibody 16131-1-APDownload protocol
IHC protocol for Citrate synthase antibody 16131-1-APDownload protocol
IF protocol for Citrate synthase antibody 16131-1-APDownload protocol
IP protocol for Citrate synthase antibody 16131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

Authors - Miranda Y Fong
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanIF

ACS Cent Sci

A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species

Authors - Cong Tang
humanIF

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
mouseWB,IF

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

RAHUL (Verified Customer) (07-03-2019)

Works very good with human cell lysates. 1:1000 dilution in 5% milk; 1 hr incubation at RT.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: DU145 prostate cancer cells