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Citrate synthase Polyclonal antibody
Citrate synthase Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 16131-1-AP
|Positive WB detected in||mouse heart tissue, HeLa cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16131-1-AP targets Citrate synthase in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Citrate synthase fusion protein Ag9117|
|Full Name||citrate synthase|
|Calculated molecular weight||466 aa, 52 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC010106|
|Gene ID (NCBI)||1431|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.
|Product Specific Protocols|
|WB protocol for Citrate synthase antibody 16131-1-AP||Download protocol|
|IHC protocol for Citrate synthase antibody 16131-1-AP||Download protocol|
|IF protocol for Citrate synthase antibody 16131-1-AP||Download protocol|
|IP protocol for Citrate synthase antibody 16131-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
RAHUL (Verified Customer) (07-03-2019)
Works very good with human cell lysates. 1:1000 dilution in 5% milk; 1 hr incubation at RT.