CSF1R Polyclonal antibody

CSF1R Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 25949-1-AP

Synonyms

C FMS, CD115, CSF 1 R, CSF1R, CSFR, FIM2, FMS, Proto oncogene c Fms



Tested Applications

Positive IHC detected inmouse brain tissue, human brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25949-1-AP targets CSF1R in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSF1R fusion protein Ag22845
Full Name colony stimulating factor 1 receptor
Calculated Molecular Weight 972 aa, 108 kDa
GenBank Accession NumberBC047521
Gene Symbol CSF1R
Gene ID (NCBI) 1436
RRIDAB_2880306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).

Protocols

Product Specific Protocols
IHC protocol for CSF1R antibody 25949-1-APDownload protocol
IF protocol for CSF1R antibody 25949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

Targeting CSF1R Alone or in Combination with PD1 in Experimental Glioma.

Authors - Justyna M Przystal
humanIHC

Arthritis Res Ther

Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies.

Authors - Ping Li
humanIHC

CNS Neurosci Ther

A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma

Authors - Wenhua Fan
humanIHC

Front Genet

Human placenta-based genome-wide mRNA sequencing to identify TEK/IGF1/CSF1/ANGPT2 as crucial segments in the pathogenesis of pre-eclampsia

Authors - Lifeng Wang
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
mouse,humanIF

Int J Exp Pathol

Inhibition of inflammation and infiltration of M2 macrophages in NSCLC through the ATF3/CSF1 axis: Role of miR-27a-3p

Authors - Bin Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jaouhara (Verified Customer) (12-05-2023)

Used in synovium PFA-fixed slides to detect macrophages. Works well.

  • Applications: Immunohistochemistry