CSNK2B Polyclonal antibody

CSNK2B Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22418-1-AP

Synonyms

CSK2B, CK2N, CK2B, CK II beta, Casein kinase II subunit beta

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Validation Data Gallery

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  WB analysis using 22418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22418-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22418-1-AP (Casein Kinase 2 beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human thyroid using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse testis using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22418-1-AP (Casein Kinase 2 beta antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22418-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22418-1-AP (CSNK2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 22418-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CSNK2B Polyclonal antibody (22418-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, NIH/3T3 cells, K-562 cells, MCF-7 cells
• Positive IHC detected in: human testis tissue, human thyroid tissue, mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

22418-1-AP targets CSNK2B in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CSNK2B fusion protein Ag18134
• Full Name: casein kinase 2, beta polypeptide
• Calculated Molecular Weight: 215 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC112017
• Gene Symbol: CSNK2B
• Gene ID (NCBI): 1460
• RRID: AB_2879102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

Publications

Species	Application	Title
mouse	WB	Biochem Biophys Res Commun; β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.; Authors - Yan Zhang
human	WB	Cancer Metab; Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1; Authors - Hiroki Okanishi
human	WB	Nat Immunol; GIMAP5 deficiency reveals a mammalian ceramide-driven longevity assurance pathway; Authors - Ann Y Park