CSNK2B Polyclonal antibody

CSNK2B Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 22418-1-AP


Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human thyroid tissue, mouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

22418-1-AP targets CSNK2B in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK2B fusion protein Ag18134
Full Name casein kinase 2, beta polypeptide
Calculated Molecular Weight 215 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC112017
Gene Symbol CSNK2B
Gene ID (NCBI) 1460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.


Product Specific Protocols
WB protocol for CSNK2B antibody 22418-1-APDownload protocol
IHC protocol for CSNK2B antibody 22418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

β-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in mouse embryonic stem cells.

Authors - Yan Zhang

Cancer Metab

Phosphoproteomics revealed cellular signals immediately responding to disruption of cancer amino acid homeostasis induced by inhibition of l-type amino acid transporter 1

Authors - Hiroki Okanishi