CSNK2B Antibody

CSNK2B Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 22418-1-AP

Synonyms

Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human thyroid tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 22418-1-AP immunogen is Fusion Protein CSNK2B protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSNK2B fusion protein Ag18134
Full Name casein kinase 2, beta polypeptide
Calculated molecular weight 215aa,25 kDa
Observed molecular weight 25-30 kDa
GenBank accession number BC112017
Gene symbol CSNK2B
Gene ID (NCBI) 1460
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.

Protocols

Product Specific Protocols
WB protocol for CSNK2B antibody 22418-1-APDownload protocol
IHC protocol for CSNK2B antibody 22418-1-APDownload protocol
FC protocol for CSNK2B antibody 22418-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols