CST4 Recombinant monoclonal antibody

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  WB analysis of human saliva using 86955-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 86955-1-RR (CST4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86955-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86955-1-RR against Human CST4 were performed. The affinity constant is 0.257 nM.

CST4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86955-1-RR

Clone No.252073B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Cystatin 4, cystatin S, Cystatin-4, Cystatin-S, Cystatin-SA-III

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Tested Applications

• Positive WB detected in: human saliva

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86955-1-RR targets CST4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3098

  Product name: recombinant human CST4 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-141 aa of NM_001899.2

  Sequence: SSSKEENRIIPGGIYDADLNDEWVQRALHFAISEYNKATEDEYYRRPLQVLRAREQTFGGVNYFFDVEVGRTICTKSQPNLDTCAFHEQPELQKKQLCSFEIYEVPWEDRMSLVNSRCQEA

• Full Name: cystatin S
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_001899.2
• Gene Symbol: Cystatin S
• Gene ID (NCBI): 1472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01036
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cystatins are a family of inhibitors of cysteine peptidases that comprises the salivary cystatins D and S-type (including CST5 and CST4,1,2) and cystatin C-type (CST3) (PMID: 25329717). The D and S-type cystatin have also been detected in seminal plasma, tears, and tracheobronchial fluid but not in other fluids and secretions where cystatin C is found. And the CST4 was expressed in the serous acinar and demilune cells of the human submandibular gland and at lower levels in the serous acini of the parotid gland (PMID:11879580). CST4 specifically combines with cysteine protease to regulate its activity, thus preventing hydrolysis of the extracellular matrix. And some studies propose that CST4 might be a biomarker for gastrointestinal cancer (PMID:29218251; PMID:29636621).