|Positive WB detected in||HeLa cells, HEK-293 cells, K-562 cells, mouse kidney tissue, HepG2 cells, mouse brain tissue|
|Positive IP detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10064-2-AP targets CSTF1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CSTF1 fusion protein Ag0109|
|Full Name||cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa|
|Calculated molecular weight||48.3 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC001011|
|Gene ID (NCBI)||1477|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.
Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.
p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.
The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.