CSTF1 Polyclonal antibody

CSTF1 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 10064-2-AP

Synonyms

CF 1 50 kDa subunit, Cleavage stimulation factor, CstF 50, CSTF 50 kDa subunit, CSTF1, CstFp50



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, K-562 cells, mouse kidney tissue, HepG2 cells, mouse brain tissue
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10064-2-AP targets CSTF1 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CSTF1 fusion protein Ag0109
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Calculated molecular weight 48.3 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC001011
Gene symbol CSTF1
Gene ID (NCBI) 1477
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage stimulation factor (CstF) , a heterotrimeric protein with subunits of 77, 64, and 50 kD (CstF-77, -64, and -50), is a polyadenylation factor that helps to specify the site of processing. CstF recognizes the G+U-rich element, a sequence located downstream of the cleavage site. CstF-50 subunit interacts with the COOH-terminal domain of the RNAP II largest subunit (CTD). This subunit may be responsible for the interaction of CSTF with other factors to form a satable complex on the pre-mRNA, and multiple WD domains are required for protein interactions.

Protocols

Product Specific Protocols
WB protocol for CSTF1 antibody 10064-2-APDownload protocol
IP protocol for CSTF1 antibody 10064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Andrology

Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.

Authors - J Dai
humanWB

Oncogene

p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.

Authors - F I Nazeer
humanWB

EMBO J

The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.

Authors - Patricia Uguen