CTSA Recombinant antibody

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  WB analysis using 80272-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80272-3-RR (CTSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80272-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80272-3-RR against Human CTSA were performed. The affinity constant is 0.717 nM.

CTSA Recombinant Antibody for WB, ELISA

Cat No. 80272-3-RR

Clone No.251086D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

cathepsin A, EC:3.4.16.5, GLB2, GSL, Lysosomal protective protein

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Tested Applications

• Positive WB detected in: A549 cells, DU 145 cells, PC-3 cells, SK-BR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80272-3-RR targets CTSA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CTSA fusion protein Ag7073
• Full Name: cathepsin A
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-60, 32, 20 kDa
• GenBank Accession Number: BC000597
• Gene Symbol: Cathepsin A
• Gene ID (NCBI): 5476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10619
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).