Cathepsin V Polyclonal antibody

Cathepsin V Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18442-1-AP

Synonyms

CTSL2, CTSV, CTSU, CATL2, Cathepsin U

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Validation Data Gallery

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  WB analysis using 18442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18442-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 18442-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 18442-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 18442-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18442-1-AP (Cathepsin V antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat liver tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

18442-1-AP targets Cathepsin V in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin V fusion protein Ag13284
• Full Name: cathepsin L2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 26-36 kDa
• GenBank Accession Number: BC023504
• Gene Symbol: Cathepsin V
• Gene ID (NCBI): 1515
• RRID: AB_10644340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Publications

Species	Application	Title
human	IHC	Mol Carcinog; Cathepsin V is correlated with the prognosis and tumor microenvironment in liver cancer; Authors - Junyu Liu