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  • KD/KO Validated

CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 12895-1-AP


CUL 1, CUL1, cullin 1

Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, K-562 cells
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12895-1-AP targets CUL1 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL1 fusion protein Ag3585
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.


Product Specific Protocols
WB protocol for CUL1 antibody 12895-1-APDownload protocol
IHC protocol for CUL1 antibody 12895-1-APDownload protocol
IP protocol for CUL1 antibody 12895-1-APDownload protocol
FC protocol for CUL1 antibody 12895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Redox Biol

Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.

Authors - Ji Zhang

Pharmacol Res

Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.

Authors - Xingda Li


A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

Authors - Muzammal Hussain

J Cell Sci

Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.

Authors - Tomoaki Nagai
  • KD Validated

Cancer Cell Int

The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.

Authors - Huilin Zhang

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


WEI (Verified Customer) (05-11-2022)

strong band for predicted band

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart