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  • KD/KO Validated

CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12895-1-AP

Synonyms

CUL 1, CUL1, cullin 1



Tested Applications

Positive WB detected inBxPC-3 cells, A549 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

12895-1-AP targets CUL1 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL1 fusion protein Ag3585
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 12895-1-APDownload protocol
IHC protocol for CUL1 antibody 12895-1-APDownload protocol
IP protocol for CUL1 antibody 12895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Cycle

Inhibition of neddylation causes meiotic arrest in mouse oocyte.

Authors - Mo Yang
  • KD Validated
humanWB

Front Bioeng Biotechnol

Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.

Authors - Kaile Zhang
humanWB

Exp Cell Res

Nedd8-activating enzyme inhibitor MLN4924 (Pevonedistat), inhibits miR-1303 to suppress human breast cancer cell proliferation via targeting p27Kip1.

Authors - Yujiao Chen
humanWB

Neoplasia

Gossypol inhibits cullin neddylation by targeting SAG-CUL5 and RBX1-CUL1 complexes.

Authors - Qing Yu
humanWB

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song
humanWB

Cancer Cell Int

The potential oncogenic and MLN4924-resistant effects of CSN5 on cervical cancer cells.

Authors - Huilin Zhang