CUL1 Polyclonal antibody

CUL1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28404-1-AP

Synonyms

CUL 1, CUL1, cullin 1



Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28404-1-AP targets CUL1 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CUL1 fusion protein Ag29145
Full Name cullin 1
Calculated molecular weight 90 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC034318
Gene symbol CUL1
Gene ID (NCBI) 8454
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protocols

Product Specific Protocols
WB protocol for CUL1 antibody 28404-1-APDownload protocol
IHC protocol for CUL1 antibody 28404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols