CXCL11 Polyclonal antibody

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  WB analysis using 10707-1-AP

  IFN gamma, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 10707-1-AP (CXCL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10707-1-AP (CXCL11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10707-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10707-1-AP (CXCL11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCL11 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10707-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CXCL-11, Interferon gamma-inducible protein 9, H174, CXCL 11, C-X-C motif chemokine 11

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Tested Applications

• Positive WB detected in: IFN gamma, LPS and Brefeldin A treated THP-1 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

10707-1-AP targets CXCL11 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL11 fusion protein Ag1087
• Full Name: chemokine (C-X-C motif) ligand 11
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC005292
• Gene Symbol: CXCL11
• Gene ID (NCBI): 6373
• RRID: AB_2088022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14625
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL11, also known as IFN-inducible T-cell α-chemoattractant (I-TAC), is a member of the ELR-negative CXC chemokine family. It is produced by various cells including leukocytes, fibroblasts, and endothelial cells upon stimulation with interferons (IFNs). CXCL11 signals through the chemokine receptors CXCR3 and CXCR7 . This chemokine is associated with multiple functions such as chemotactic migration, regulation of cell proliferation and self-renewal, increasing cell adhesion, and modulation of angiostatic effects.

Publications

Species	Application	Title
human	IHC	Biomedicines; Identification and Preliminary Clinical Validation of Key Extracellular Proteins as the Potential Biomarkers in Hashimoto's Thyroiditis by Comprehensive Analysis; Authors - Zihan Xi
human	IHC	Cancer Immunol Immunother; Comprehensive analysis of the relationship between ubiquitin-specific protease 21 (USP21) and prognosis, tumor microenvironment infiltration, and therapy response in colorectal cancer; Authors - Haihang Nie
		Signal Transduct Target Ther; Ultra-high dose rate radiotherapy overcomes radioresistance in head and neck squamous cell carcinoma; Authors - Hong-Shuai Li