CXCL12/SDF-1 Antibody

CXCL12/SDF-1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17402-1-AP

Synonyms

C X C motif chemokine 12, CXCL12, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1



Tested Applications

Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 17402-1-AP immunogen is Fusion Protein CXCL12/SDF-1 protein expressed in E. coli.

Reactivity human
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CXCL12/SDF-1 fusion protein Ag11379
Full Name chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
Calculated molecular weight 89aa,10 kDa
Observed molecular weight
GenBank accession number BC039893
Gene symbol CXCL12
Gene ID (NCBI) 6387
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.

Protocols

Product Specific Protocols
IHC protocol for CXCL12/SDF-1 antibody 17402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Int J Oncol

MACC1 overexpression in carcinoma‑associated fibroblasts induces the invasion of lung adenocarcinoma cells via paracrine signaling.

Authors - Zhuoshi Li
rat

Eur Neuropsychopharmacol

Effect of chronic methylphenidate treatment on hippocampal neurovascular unit and memory performance in late adolescent rats.

Authors - Vanessa Coelho-Santos
mouseIF

Int Immunopharmacol

High-mobility group box-1-Toll-Like receptor 4 axis mediates the recruitment of endothelial progenitor cells in alkali-induced corneal neovascularization.

Authors - Shuai Yang
mouseIF

Neuroscience

Effects of crenolanib, a nonselective inhibitor of PDGFR, in a mouse model of transient middle cerebral artery occlusion.

Authors - Jianping Wang
mouseWB

Int J Hyperthermia

The cryo-thermal therapy-induced IL-6-rich acute pro-inflammatory response promoted DCs phenotypic maturation as the prerequisite to CD4(+) T cell differentiation.

Authors - Kun Liu
humanIHC

Sci Rep

Bioinformatic analysis reveals the importance of epithelial-mesenchymal transition in the development of endometriosis.

Authors - Meihong Chen