CXCL12/SDF-1 Polyclonal antibody
CXCL12/SDF-1 Polyclonal Antibody for IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 17402-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 6 publications below |
IHC | See 3 publications below |
IF | See 6 publications below |
Product Information
The immunogen of 17402-1-AP is CXCL12/SDF-1 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CXCL12/SDF-1 fusion protein Ag11379 |
Full Name | chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) |
Calculated molecular weight | 89 aa, 10 kDa |
GenBank accession number | BC039893 |
Gene symbol | CXCL12 |
Gene ID (NCBI) | 6387 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.
Protocols
Product Specific Protocols | |
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IHC protocol for CXCL12/SDF-1 antibody 17402-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Oncol MACC1 overexpression in carcinoma‑associated fibroblasts induces the invasion of lung adenocarcinoma cells via paracrine signaling. | ||
Int Immunopharmacol High-mobility group box-1-Toll-Like receptor 4 axis mediates the recruitment of endothelial progenitor cells in alkali-induced corneal neovascularization. | ||
Cell Biol Int The protective effects of umbilical cord-derived endothelial colony forming cells on hepatic veno-occlusive disease. | ||
Future Oncol Cancer-associated fibroblasts induce epithelial-mesenchymal transition and cisplatin resistance in ovarian cancer via CXCL12/CXCR4 axis. | ||
Neuroscience Effects of crenolanib, a nonselective inhibitor of PDGFR, in a mouse model of transient middle cerebral artery occlusion. |