CXCL12/SDF-1 Antibody
CXCL12/SDF-1 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, ELISA
Conjugate
Unconjugated
Cat no : 17402-1-AP
Synonyms
C X C motif chemokine 12, CXCL12, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
The 17402-1-AP immunogen is Fusion Protein CXCL12/SDF-1 protein expressed in E. coli.
| Reactivity | human |
| Cited Species | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CXCL12/SDF-1 fusion protein Ag11379 |
| Full Name | chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) |
| Calculated molecular weight | 89aa,10 kDa |
| Observed molecular weight | |
| GenBank accession number | BC039893 |
| Gene symbol | CXCL12 |
| Gene ID (NCBI) | 6387 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20oC. Aliquoting is unnecessary for -20oC storage. |
Background Information
In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CXCL12/SDF-1 antibody 17402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Oncol MACC1 overexpression in carcinoma‑associated fibroblasts induces the invasion of lung adenocarcinoma cells via paracrine signaling. | ||
Eur Neuropsychopharmacol Effect of chronic methylphenidate treatment on hippocampal neurovascular unit and memory performance in late adolescent rats. | ||
Int Immunopharmacol High-mobility group box-1-Toll-Like receptor 4 axis mediates the recruitment of endothelial progenitor cells in alkali-induced corneal neovascularization. | ||
Neuroscience Effects of crenolanib, a nonselective inhibitor of PDGFR, in a mouse model of transient middle cerebral artery occlusion. | ||
Int J Hyperthermia The cryo-thermal therapy-induced IL-6-rich acute pro-inflammatory response promoted DCs phenotypic maturation as the prerequisite to CD4(+) T cell differentiation. | ||
Sci Rep Bioinformatic analysis reveals the importance of epithelial-mesenchymal transition in the development of endometriosis. |
