CXCL17 Polyclonal antibody

CXCL17 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18108-1-AP

Synonyms

Dendritic cell and monocyte chemokine-like protein, Dcip1, C-X-C motif chemokine 17, C X C motif chemokine 17, 6-Cys CXCL17

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Validation Data Gallery

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  IHC staining of human colon cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18108-1-AP (CXCL17 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18108-1-AP (CXCL17 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18108-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18108-1-AP (CXCL17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

18108-1-AP targets CXCL17 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL17 fusion protein Ag12516
• Full Name: chemokine (C-X-C motif) ligand 17
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC093946
• Gene Symbol: CXCL17
• Gene ID (NCBI): 284340
• RRID: AB_2878502
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCL17, also known as C-X-C motif chemokine 17, is a relatively newly discovered member of the CXC chemokine family, which plays a multifaceted role in immune responses and other biological processes. CXCL17 has been implicated in several human pathologies, and its role in mediating immune responses is of particular interest. It is involved in the recruitment of immune cells, angiogenesis, and control of microorganisms at mucosal barriers . It is also known to be involved in tumor angiogenesis and has shown both proinflammatory and anti-inflammatory effects. CXCL17 is highly expressed in the gastric mucosa and other mucosal tissues. Its receptor was identified as GPR35 and named CXCR8, although the functional role of this interaction is not yet fully understood. CXCL17's expression is associated with both disease progression and protection in various diseases. It has been linked to pulmonary fibrosis, asthma, lung cancer, and hepatic cancer, where increased expression is associated with disease progression. Conversely, it may play a protective role in pancreatic cancer, autoimmune encephalomyelitis, and viral infections. Research has shown that CXCL17 promotes neutrophil trafficking and plays a role in the early proinflammatory response by facilitating the recruitment of neutrophils to the site of insult . It also exhibits chemoattractant abilities targeting monocytes and macrophages and can induce the production of proangiogenic factors such as vascular endothelial growth factor A from treated monocytes

Publications

Species	Application	Title
human	IHC	Nat Commun; Dissecting esophageal squamous-cell carcinoma ecosystem by single-cell transcriptomic analysis.; Authors - Xiannian Zhang
human	IHC, WB, IF	J Dermatol Sci; CXCL17-mediated downregulation of type I collagen via MMP1 and miR-29 in skin fibroblasts possibly contributes to the fibrosis in systemic sclerosis.; Authors - Shuichi Shimada
human	IHC	Cancers (Basel); A Novel Risk Score Model of Lactate Metabolism for Predicting over Survival and Immune Signature in Lung Adenocarcinoma; Authors - Zhou Jiang
human	WB	Metab Brain Dis; Sequencing and Bioinformatics analysis of lncRNA/circRNA-miRNA-mRNA in Glioblastoma multiforme; Authors - Renjie Wang