CXCR4 Monoclonal antibody

• Featured Product
• KD/KO Validated

CXCR4 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

35

CloneNo.

4B5E4

Cat no : 60042-1-Ig

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

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Validation Data Gallery

• 

  WB analysis of HeLa using 60042-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 60042-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 60042-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60042-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60042-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 60042-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60042-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 60042-1-Ig

  1X10^6 Jurkat cells were stained with 0.2ug CXCR4 antibody (60042-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, Daudi cells, Ramos cells, Jurkat cells, U-937 cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue, mouse brain tissue, human prostate cancer tissue, human breast cancer tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 8 publications below
• WB: See 31 publications below
• IHC: See 8 publications below
• IF: See 8 publications below
• CoIP: See 2 publications below

Product Information

60042-1-Ig targets CXCR4 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: CXCR4 fusion protein Ag1528
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated molecular weight: 352 aa, 40 kDa
• Observed molecular weight: 60~70 kDa
• GenBank accession number: BC020968
• Gene symbol: CXCR4
• Gene ID (NCBI): 7852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Publications

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
mouse	WB	Nat Commun; HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity.; Authors - Hiroaki Semba
mouse	WB,IF	J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando ; KD Validated
human	WB	Nanoscale; Mitomycin C-treated human-induced pluripotent stem cells as a safe delivery system of gold nanorods for targeted photothermal therapy of gastric cancer; Authors - Meng Yang
human	WB	ACS Appl Mater Interfaces; Surface Functionalization of Polymeric Nanoparticles with Umbilical Cord-Derived Mesenchymal Stem Cell Membrane for Tumor-Targeted Therapy.; Authors - Na Yang
human,mouse	WB,IHC,IF	Int J Cancer; Tissue-resident CXCR4+ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression; Authors - Zhenyu Liao ; KO Validated

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