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  • KD/KO Validated

CXCR4 Monoclonal antibody

CXCR4 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

4B5E4

Cat no : 60042-1-Ig

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Daudi cells, Ramos cells, Jurkat cells, U-937 cells, THP-1 cells
Positive IHC detected inhuman tonsillitis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

60042-1-Ig targets CXCR4 in WB, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen CXCR4 fusion protein Ag1528
Full Name chemokine (C-X-C motif) receptor 4
Calculated molecular weight 352 aa, 40 kDa
Observed molecular weight 60~70 kDa
GenBank accession numberBC020968
Gene symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Protocols

Product Specific Protocols
WB protocol for CXCR4 antibody 60042-1-IgDownload protocol
IHC protocol for CXCR4 antibody 60042-1-IgDownload protocol
FC protocol for CXCR4 antibody 60042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

The overexpression of GRASP might inhibit cell proliferation and invasion in hepatocellular carcinoma.

Authors - Yang Cheng
mouseWB

Evid Based Complement Alternat Med

Combination of Ligusticum Chuanxiong and Radix Paeonia Promotes Angiogenesis in Ischemic Myocardium through Notch Signalling and Mobilization of Stem Cells.

Authors - Wei-Li Shi
humanWB

Cell Death Dis

Cancer-associated fibroblast-derived SDF-1 induces epithelial-mesenchymal transition of lung adenocarcinoma via CXCR4/β-catenin/PPARδ signalling.

Authors - Yingyan Wang
  • KD Validated
humanWB, IF, CoIP

Cell Death Discov

Fibroblast CEBPD/SDF4 axis in response to chemotherapy-induced angiogenesis through CXCR4.

Authors - Jhih-Ying Chi
humanWB,IHC

Cell Death Discov

ETV4 promotes breast cancer cell stemness by activating glycolysis and CXCR4-mediated sonic Hedgehog signaling.

Authors - Tao Zhu
  • KD Validated
WB

Mol Med Rep

Taspine derivative TAS9 regulates cell growth and metastasis of human hepatocellular carcinoma.

Authors - Rui Liu