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CXCR7 Monoclonal antibody

CXCR7 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

4C3D7

Cat no : 60216-1-Ig

Synonyms

ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CXCR7 Monoclonal antibody (60216-1-Ig)

    WB analysis using 60216-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60216-1-Ig (CXCR7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human prostate cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, U-87 MG cells, Jurkat cells, Raji cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, human prostate cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

60216-1-Ig targets CXCR7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CXCR7 fusion protein Ag14247
Full Name chemokine (C-X-C motif) receptor 7
Calculated Molecular Weight 362 aa, 41 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC036661
Gene Symbol CXCR7
Gene ID (NCBI) 57007
RRIDAB_11182925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

Protocols

Product Specific Protocols
WB protocol for CXCR7 antibody 60216-1-IgDownload protocol
IHC protocol for CXCR7 antibody 60216-1-IgDownload protocol
IF protocol for CXCR7 antibody 60216-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Urol Oncol

The inflammation-related biomarker CXCR7 independently predicts patient outcome after radical prostatectomy.

Authors - Pedro Bargão Santos
View Article