NOX2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NOX2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

140

Cat no : 19013-1-AP

Synonyms

CGD, CGD91 phox, CYBB, Cytochrome b 245 heavy chain, Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, GP91 1, GP91 PHOX, GP91PHOX, NADPH oxidase 2, NOX2, p22 phagocyte B cytochrome, p91 PHOX

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Validation Data Gallery

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  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19013-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 19013-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19013-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NOX2 antibody (19013-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 19013-1-AP (NOX2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of RAW 264.7 using 19013-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human NOX2 (19013-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Raji cells, human plasma, Jurkat cells, mouse brain tissue, rat spleen tissue
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, human tonsillitis tissue
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 122 publications below
• IHC: See 14 publications below
• IF: See 11 publications below

Product Information

19013-1-AP targets NOX2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOX2 fusion protein Ag5536
• Full Name: cytochrome b-245, beta polypeptide
• Calculated molecular weight: 577 aa, 65 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC032720
• Gene symbol: CYBB
• Gene ID (NCBI): 1536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reveals the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.

Publications

Species	Application	Title
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
mouse	WB,IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
pig	IF,WB	Redox Biol; Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.; Authors - Chengjun Hu ; KD Validated
rat	WB	Redox Biol; RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.; Authors - Nan Wu
human	WB	Theranostics; Targeting Epstein-Barr virus oncoprotein LMP1-mediated high oxidative stress suppresses EBV lytic reactivation and sensitizes tumors to radiation therapy.; Authors - Jianmin Hu ; KD Validated