NOX2 Polyclonal antibody

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  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 19013-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 19013-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19013-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19013-1-AP (NOX2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19013-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19013-1-AP (NOX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 19013-1-AP (NOX2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse spleen using 19013-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using NOX2 antibody (19013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of RAW 264.7 using 19013-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human NOX2 (19013-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of MCF-7 using 19013-1-AP

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug NOX2 Polyclonal antibody (19013-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

NOX2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 19013-1-AP

Publications(199)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, FC (Intra), CoIP, ELISA

Synonyms

CYBB, CGD, CGD91-phox, Cytochrome b-245 heavy chain, EC:1.-.-.-

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Jurkat cells, Raji cells, human plasma, rat spleen tissue
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue, human tonsillitis tissue
• Positive FC (Intra) detected in: RAW 264.7 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 173 publications below
• IHC: See 20 publications below
• IF: See 23 publications below
• CoIP: See 1 publications below

Product Information

19013-1-AP targets NOX2 in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NOX2 fusion protein Ag5536
• Full Name: cytochrome b-245, beta polypeptide
• Calculated Molecular Weight: 577 aa, 65 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC032720
• Gene Symbol: NOX2
• Gene ID (NCBI): 1536
• RRID: AB_2833044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04839
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Cell Host Microbe; Zinc promotes microbial p-coumaric acid production that protects against cholestatic liver injury; Authors - Dongping Li
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
mouse	WB,IHC	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
mouse	WB	Brain Behav Immun; Adiponectin ameliorates traumatic brain injury-induced ferroptosis through AMPK- ACC1 signaling pathway; Authors - Yufeng Ge
		Sci Adv; Sphingolipid biosynthesis is essential for metabolic rewiring during TH17 cell differentiation; Authors - Thiruvaimozhi Abimannan
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen