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NOX2 Polyclonal antibody

NOX2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 19013-1-AP


CYBB, Cytochrome b 245 heavy chain, NOX2, p91 PHOX

Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, Raji cells, human plasma, mouse brain tissue
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19013-1-AP is NOX2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity canine, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOX2 fusion protein Ag5536
Full Name cytochrome b-245, beta polypeptide
Calculated molecular weight 577 aa, 65 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC032720
Gene symbol CYBB
Gene ID (NCBI) 1536
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reveals the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.


Product Specific Protocols
WB protocol for NOX2 antibody 19013-1-APDownload protocol
IHC protocol for NOX2 antibody 19013-1-APDownload protocol
IF protocol for NOX2 antibody 19013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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