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NOX2 Polyclonal antibody

NOX2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19013-1-AP

Synonyms

CYBB, Cytochrome b 245 heavy chain, NOX2, p91 PHOX



Tested Applications

Positive WB detected inmouse spleen tissue, mouse brain tissue, Raji cells, human plasma tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19013-1-AP is NOX2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOX2 fusion protein Ag5536
Full Name cytochrome b-245, beta polypeptide
Calculated molecular weight 577 aa, 65 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC032720
Gene symbol CYBB
Gene ID (NCBI) 1536
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781). In our results, it reveals the bands of 55-65 kDa and 80 kDa besides a band of 140 kDa that we do not find the reason.

Protocols

Product Specific Protocols
WB protocol for NOX2 antibody 19013-1-APDownload protocol
IHC protocol for NOX2 antibody 19013-1-APDownload protocol
IF protocol for NOX2 antibody 19013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Free Radic Biol Med

Inhibiting inflammation and modulating oxidative stress in oxalate-induced nephrolithiasis with the Nrf2 activator dimethyl fumarate.

Authors - Jianning Zhu
ratWB

J Cell Physiol

Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.

Authors - Pei Liu
ratWB

Asian J Androl

Human tissue kallikrein-1 protects against the development of erectile dysfunction in a rat model of hyperhomocysteinemia.

Authors - Kai Cui
ratWB

Front Pharmacol

Protective Effects of 18β-Glycyrrhetinic Acid on Monocrotaline-Induced Pulmonary Arterial Hypertension in Rats.

Authors - Min Zhang
humanWB

Oxid Med Cell Longev

BCL6 Attenuates Proliferation and Oxidative Stress of Vascular Smooth Muscle Cells in Hypertension.

Authors - Dan Chen
ratWB

J Physiol

Chronic fetal hypoxia disrupts the peri-conceptual environment in next-generation adult female rats.

Authors - C E Aiken