CYP4F2-Specific Polyclonal antibody, PBS Only

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  WB analysis of A549 using 20010-1-AP (same clone as 20010-1-PBS)

  WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20010-1-AP (same clone as 20010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CYP4F2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20010-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

20010-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: NM_001082
• Gene Symbol: CYP4F2
• Gene ID (NCBI): 8529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78329
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.