CYP4F2-Specific Polyclonal antibody

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• KD/KO Validated

CYP4F2-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20010-1-AP

Synonyms

CPF2, CYP4F2, CYP4F2-Specific, CYPIVF2, Cytochrome P450 4F2, Cytochrome P450 LTB omega

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Validation Data Gallery

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  WB analysis using 20010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20010-1-AP

  WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells.

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  IHC staining of human liver using 20010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HuH-7 cells, L02 cells, SMMC-7721 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20010-1-AP targets CYP4F2-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 4, subfamily F, polypeptide 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50-53 kDa
• GenBank Accession Number: NM_001082
• Gene Symbol: CYP4F2
• Gene ID (NCBI): 8529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.