CYP7A1 Polyclonal antibody

• 

  WB analysis using 18054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18054-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18054-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CYP7A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18054-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

---

Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, L02 cells, SMMC-7721 cells
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 4 publications below

Product Information

18054-1-AP targets CYP7A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12572

  Product name: Recombinant human CYP7A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 154-504 aa of BC101777

  Sequence: RPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

• Full Name: cytochrome P450, family 7, subfamily A, polypeptide 1
• Calculated Molecular Weight: 504 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC101777
• Gene Symbol: CYP7A1
• Gene ID (NCBI): 1581
• RRID: AB_3085551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22680
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Mol Gastroenterol Hepatol; Endoplasmic Reticulum Stress Regulates Hepatic Bile Acid Metabolism in Mice.; Authors - Anne S Henkel
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
mouse	IHC	Ecotoxicol Environ Saf; Triclosan exposure causes abnormal bile acid metabolism through IL-1β-NF-κB-Fxr signaling pathway; Authors - Jing Liu
mouse	WB	J Ethnopharmacol; Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice; Authors - Si-Wei Rao
MOUSE	WB	J Lipid Res; Hepatic Deletion of X-box Binding Protein 1 Impairs Bile Acid Metabolism in Mice.; Authors - Xiaoying Liu
mouse	WB	Toxicol Sci; Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.; Authors - Disi Bai